Assessment of morphological and genetic diversity in <Emphasis Type="Italic">Gmelina arborea</Emphasis> Roxb. |
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Authors: | Dhiraj Naik Durgeshwer Singh Varsha Vartak Sharayu Paranjpe Sujata Bhargava |
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Institution: | (1) Botany Department, University of Pune, Pune, 411 007, India;(2) Statistics Department, University of Pune, Pune, 411 007, India;(3) Department of Biology, West Virginia University, 53 Campus Dr., P. O. Box 6057, Morgantown, WV 26506, USA |
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Abstract: | Gmelina arborea is an important timber-yielding tree that grows naturally in the tropical and subtropical regions of Southeast Asia and has
also been introduced as a plantation species outside these regions. Genetic diversity in this tree species was observed in
stone/seed-related traits and in vitro responses of cultured nodal segments from plants of eight different populations representing
natural forests, fragmented forests and plantations. Variance analysis showed significant differences between populations
for these traits. However, it was not possible to separate the different populations using these traits by multivariate analysis,
even after environmental variation was reduced over six subcultures. Genetic diversity was therefore analysed using molecular
markers. Inter-simple sequence repeat (ISSR) primers yielded 95% polymorphic loci among the eight populations and UPGMA analysis
enabled separation of these populations on the basis of their genetic distances. Diversity was also analyzed using population
genetics parameters like Nei’s genetic diversity and gene differentiation. Nei’s genetic diversity was 0.29 between populations
and 0.11 within populations. AMOVA analysis indicated 41 and 59% within- and between-population genetic diversity, respectively.
Mantel test suggested that genetic differentiation between six Indian populations was positively correlated to geographic
distance (r = 0.626, P = 0.029). Assessment of the genetic variation in G. arborea populations is an important step in selection of conservation strategies for this species since diversity forms the basis
for species adaptation. |
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Keywords: | ISSR Gmelina arborea Genetic variation Provenances Tissue culture |
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