| 用激光扫描共聚焦显微镜观测果树胚胎特征的方法 |
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| 引用本文： | 商月惠,董文轩,王颖,王蒴,于维军,周攀. 用激光扫描共聚焦显微镜观测果树胚胎特征的方法[J]. 安徽农业科学, 2007, 35(14): 4097-4098,4147. DOI: 10.3969/j.issn.0517-6611.2007.14.008 |
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| 作者姓名： | 商月惠 董文轩 王颖 王蒴 于维军 周攀 |
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| 作者单位： | 沈阳农业大学园艺学院,辽宁沈阳,110161;沈阳农业大学仪器分析测试中心,辽宁沈阳,110161 |
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| 基金项目： | 国家自然科学基金资助项目（30370985）. |
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| 摘 要： | 以平邑甜茶[Malus hupehensis(Pamp.)Rehd.var.pingyiensis Jiang]及其与扎矮山定子[Malus.baccata(L.)Borkh.]杂交获得的非整倍体植株为试材,采取不同的前处理方法和观测方式探索其对试材的胚胎特别是胚囊观测效果的影响.结果表明:用浓度4%蔗糖曙红溶液对胚珠进行整体染色,染色后充分水洗,最后采用LSCM 1 024×1 024的分辨率观测,获得了最清晰的胚胎特征图像.
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| 关 键 词： | 激光扫描共聚焦显微镜 胚胎 平邑甜茶 非整倍体植株 苹果属 |
| 文章编号： | 0517-6611（2007）14-04097-02 |
| 收稿时间： | 2007-01-18 |
| 修稿时间： | 2007-01-18 |
Methods for Observing Embryo Characteristics of Fruit Trees by Laser Scanning Confocal Microscope |
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| SHANG Yue-hui et al. Methods for Observing Embryo Characteristics of Fruit Trees by Laser Scanning Confocal Microscope[J]. Journal of Anhui Agricultural Sciences, 2007, 35(14): 4097-4098,4147. DOI: 10.3969/j.issn.0517-6611.2007.14.008 |
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| Authors: | SHANG Yue-hui et al |
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| Affiliation: | College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110161 |
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| Abstract: | Effects of different pretreatments and observation modes on embryo characteristics,especially the embryo sac,were studied using Malus hupehensis and the aneuploid plant from the hybridization of Malus hupehensis and Malus.baccata as experimental materials.Result showed that the clearest images of embryo characteristics could be obtained by using 4% sucrose-eosin dyestuff to stain the whole ovule,then washing the materials sufficiently and choosing LSCM 1024×1024 image resolution to observe. |
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| Keywords: | Laser scanning confocal microscope(LSCM) Embryo Malus hupehensis Aneuploid plant Malus |
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