影响产氢发酵细菌B49产氢的部分因子研究

采用间歇培养实验,研究了部分因子碳源葡萄糖、氮源、菌龄、温度及pH值对产氢发酵细菌新菌种B49(Hydrogen-producingBacterialB49,AF481148inEMBL,简写HPBB49)生物产氢的影响。试验结果表明,以葡萄糖为碳源,其浓度10g.L-1时,HPBB49的葡萄糖利用率为100%,氢气产率为1.69molH2.mol-1葡萄糖;HPBB49不能利用无机氮源,有机氮是HPBB49生长、产氢的适宜氮源;菌龄影响HPBB49的产氢;B49产氢量随细菌生长OD值的增加而增加;HPBB49生长和产氢适宜温度均为35℃;B49最适生长的pH值约为4.5,最适产氢的pH值约为4.0。

Effects of partial factors on hydrogen production by fermentative hydrogen-producing bacterial strain B49

Batch culture were employed to investigate effects of partial factors,such as carbon source of glucose,nitrogen sources,preculture time,temperature,and pH value,on hydrogen production by a novel fermentative hydrogen-producing bacterial strain B49(AF481148 in EMBL,HPB B49).Glucose consumption and hydrogen yield with 10 g·L-1 glucose as carbon source were 100% and 1.69 mol H2·mol-1 glucose,respectively.B49 could produce hydrogen only using organic nitrogen source.The hydrogen production by HPB B49 was enhanced with increase in the biomass.The preculture time could affect hydrogen production.The favorable temperature for cell growth and hydrogen production was 35 ℃.The optimum pH for cell growth and hydrogen production was around 4.5 and 4.0,respectively.