四倍体刺槐组织培养技术研究

四倍体刺槐Robinia pseudoacacia是我国引种驯化历史较久的外来树种,为人工诱交植株,分速生型和饲料型。与普通刺槐相比具有更强的适生能力,对土质要求不严格,耐干旱瘠薄,在海拔2 000 m以下、降水量不低于200 mm、年平均气温不低于3℃的地区均能生长。本实验对速生型四倍体刺槐进行了组织培养及健化技术的研究,在不定芽的诱导中,以MS、1／ 2(N)MS、LS、1／2(N)LS为基本培养基,在各培养基中,以MS+BAP0．2 mg／L+NAA0．05 mg／L的组合生长情况最好,但随着继代次数的增加,其细胞分裂素要逐步降低。在生根培养中,以1／2MS+NAA0．05 mg／L+IBAO．01 mg／L生根最好。在苗木健化中,其成活率达 73．4％。

Study on Tissue Culture Technique for Tetraploid Robinia Pseudoacacia

Robinia pseudoacacia, a domesticated exotic species with a long history, is an artificially induced variation divided into fast growing and fodder types. Compared with ordinary Robinia pseudoacacia, it is drought and sterile resistant, not restrictive to soil quality, and more adaptable, which can survive below altitude of 2 000 m. rainfall not below 200 mm and average temperature per year not below 3 ℃.This test has studied the tissue culture and exuberance technique for Robinia pseudoacacia of the fast growing type. In inducement test of adventitious bud, MS、1/2(N)MS、LS、/2(N)LS were taken as basic culture medium, among which the MS+BAP0. 2 mg/L+NAA0.05 mg/L combination turned the best growing condition. But with the increase of filial generation, their cell division would turn down step by step. Among the root rainning test, 1/2MS+NAA0.05 mg/L+IBA0.01 mg/L turned the best condition. In the seedling exuberance test, its survival rate was 73. 4%.