Mycoplasma hyopneumoniae infection in pigs: Duration of the disease and evaluation of four diagnostic assays |
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| Institution: | 1. The Federation of Danish Pig Producers and Slaughterhouses, Maglegårdsvej 2, DK-4000 Roskilde, Denmark;2. Danish Veterinary Laboratory, DK-1790 Copenhagen V, Denmark;1. Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA;2. The ithree Institute, University of Technology Sydney, Sydney, NSW, Australia;1. Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Brazil;2. Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Brazil;3. Laboratório de Imunologia Celular, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul, Brazil;4. Laboratório de Microrganismos Diazotróficos, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Brazil;1. Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA;2. Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA;3. Zoetis, Parsippany, NJ, USA;4. Swine Disease Eradication Center, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA;1. French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Ploufragan-Plouzané-Niort Laboratory, Mycoplasmology, Bacteriology and Antimicrobial Resistance Unit, Ploufragan, France;2. French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Ploufragan-Plouzané-Niort Laboratory, SPF Pig Production and Experimental Unit, Ploufragan, France;3. Bretagne Loire University, Rennes, France;1. NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568, Australia;2. School of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia;3. School of Chemistry and Molecular Biosciences and Australian Infectious Diseases Research Centre, University of Queensland, Brisbane, QLD 4072, Australia;4. The ithree Institute, University of Technology Sydney, Sydney, NSW 2007, Australia;1. Veterinary Diagnostic and Population Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA;2. Departamento de Clínica e Cirurgia Veterinárias, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;3. Independent Swine Health Consultant, Barcelona, 08195, Spain;4. Swine Vet Center, P.A., St. Peter, MN, USA;5. Swine Medicine Education Center, College of Veterinary Medicine, Iowa State University, Ames, IA, USA;6. Boehringer Ingelheim Animal Health US Inc., Atlanta, GA, USA;7. Pig Improvement Company, PIC®, Hendersonville, TN, USA |
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| Abstract: | 200 SPF pigs were infected by aerosol with Mycoplasma hyopneumoniae and the development of clinical signs, serological and pathological reactions were studied. Mean time to onset of coughing was 13 days. A mean delay of 9 days was observed from onset of coughing until seroconversion against M. hyopneumoniae as measured by ELISA. At an individual level, the sensitivity for this ELISA was estimated to 98–100% and the specificity to 93–100%. Pasteurella multocida was isolated from the majority of the lungs 4 weeks post inoculation with M. hyopneumoniae and the lung lesions in pigs were significantly larger when P. multocida was present as compared to pigs with M. hyopneumoniae alone. An evaluation of cultivation, immunofluorescence, ELISA and polymerase chain reaction for demonstration of M. hyopneumoniae in lungs showed that all four methods have a high sensitivity in the acute stages of pneumonia. In the later stages the sensitivity of cultivation was superior to the other methods. No differences in specificity were observed between the methods. The antigen-ELISA OD values and the immunofluorescence scores revealed a strong positive correlation. Nasal swabs were additionally used for demonstration of M. hyopneumoniae and the polymerase chain reaction was found superior to the other methods. |
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