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Serological characterization of Actinobacillus pleuropneumoniae biotype 2 strains isolated from pigs in two Danish herds
Institution:1. Danish Veterinary Laboratory, Bülowsvej 27, DK-1790 Copenhagen V, Denmark;2. Stenstrup Dyreklinik, DK-5771 Stenstrup, Denmark;1. Departamento de Sanidad Animal, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, 14071 Córdoba, Spain;2. CICAP—Food Research Centre, Córdoba, Pozoblanco,14400, Spain;3. Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, 14071 Córdoba, Spain;4. Technical University of Denmark, National Veterinary Institute, Lindholm, Denmark;1. Division of Respiratory Medicine, Department of Medicine, University of Malaya, Kuala Lumpur, Malaysia;2. Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;1. Department of Pharmacology, Federal University of Paraná, Curitiba, PR, Brazil;2. Department of Veterinary Medicine, Federal University of Paraná, Curitiba, PR, Brazil;3. Department of Medical Pathology, Federal University of Paraná, Curitiba, PR, Brazil;4. Department of Chemistry, Federal University of Paraná, Curitiba, PR, Brazil;5. Tytgat Institute for Liver and Intestinal Research, University of Amsterdam, Amsterdam, The Netherlands;1. Department of Veterinary Medicine Ahmadu Bello University, Zaria, Nigeria;2. Department of Veterinary Microbiology Ahmadu Bello University, Zaria, Nigeria;3. Department of Veterinary Medicine Usmanu Danfodiyo University, Sokoto, Nigeria;4. Microbiology Division, Central Diagnostic Laboratory National Veterinary Research Institute, Vom Plateau state, Nigeria;5. Department of Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria, Nigeria;6. Harper and Keele Veterinary School, Keele, Newcastle, England, UK;7. Royal Veterinary College, Pathobiology and Population Sciences, Hawkshead Lane, England, UK;1. Department of Molecular Microbiology and Immunology, University of Texas at San Antonio, San Antonio, TX, United States;2. Marine Biomedical Research Lab & Environmental Toxicology Unit, Cellular and Molecular Research Centre, Saveetha Dental College and Hospitals, Saveetha Institute of Medical & Technical Sciences, Saveetha University, Chennai 600077, Tamil Nadu, India;3. Department of Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical & Technical Sciences, Saveetha University, Chennai 600077, Tamil Nadu, India;1. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, Queensland 4067, Australia;2. Biosecurity Sciences Laboratory, Department of Agriculture and Fisheries, Coopers Plains, Queensland 4108, Australia
Abstract:Eight Actinobacillus pleuropneumoniae biotype 2 strains were isolated in pure culture from lungs of pigs originating from two Danish herds with growing and finishing pigs. The antigenic properties were studied by indirect haemagglutination (IHA) and immunodiffusion (ID) tests using soluble surface antigens and by enzyme-linked immunosorbent assays (ELISA) using capsular enriched fractions and LPS. In all tests the strains proved antigenically homogeneous and serologically distinct from the known biotype 1 and 2 serotypes. Thus, the strains represent a new serotype which is provisionally proposed as biotype 2 serotype 14.
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