Bacteriological and molecular typing of Clostridium
perfringens strains isolated in retail beef in Beijing,China |
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| Authors: | Hui JIANG Yu-Ming QIN Xiao-Tong YANG Qiao-Ling LI Qing-Chun SHEN Jia-Bo DING Run-Yu WEI Jian-Dong ZHANG Jia-Li SUN Ming-Jun SUN Xue-Zheng FAN |
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| Institution: | 1)Department of Veterinary Technology, China Institute of Veterinary Drug Control, Beijing, 100081, China;2)Laboratory of Zoonoses, China Animal Health and Epidemiology Center, Qingdao, 266032, China |
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| Abstract: | Clostridium perfringens is an important zoonotic pathogen. This study was designed to explore the prevalence and toxin types of C. perfringens in retail beef collected from Beijing, China. Among 221 beef samples collected, 53 samples were positive for C. perfringens, resulting in the average prevalence as 23.98%. By toxin gene-based typing, the most C. perfringens strains belong to type A (96.23%, 51/53), only 2 strains were identified as type D. By a multi-locus sequence typing (MLST)-based analysis, a total of 36 sequence types (STs) were detected, and the most STs (n=30) represented just a single strain. These finding suggested that the prevalence of C. perfringens in retail beef in Beijing was considerably high and these bacteria displayed extreme diversity in genetics. |
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| Keywords: | Clostridium perfringens multi-locus sequence typing retail beef toxin type |
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