Genetic stability of the open reading frame 2 (ORF2) of borna disease virus 1
(BoDV-1) distributed in cattle in Hokkaido |
| |
| Authors: | Manakorn SUKMAK Minoru OKAMOTO Tatsuya ANDO Katsuro HAGIWARA |
| |
| Affiliation: | 1)Department of Farm Resources and Production Medicine, Faculty of Veterinary Medicine, Kasetseart University, Kamphaeng Saen, 73140, Thailand;2)Kamphaengsaen Veterinary Diagnostic Center (KVDC), Faculty of Veterinary Medicine, Kasetseart University, Kamphaeng Saen, 73140, Thailand;3)School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido 069-8501, Japan |
| |
| Abstract: | Borna disease virus (BoDV) is a neurotropic virus that causes several infections in humans and neurological diseases in a wide range of animals worldwide. BoDV-1 has been molecularly and serologically detected in many domestic and wild animals in Japan; however, the genetic diversity of this virus and the origin of its infection are not fully understood. In this study, we investigated BoDV-1 infection and genetic diversity in samples collected from animals in Hokkaido between 2006 and 2020. The analysis was performed by focusing on the P region of BoDV-1 for virus detection. The presence of BoDV-1 RNA was observed in samples of brain tissue and various organs derived from persistently infected cattle. Moreover, after inoculation, BoDV-positive brains were isolated from neonatal rats. The gene sequences of the P region of BoDV obtained from the rat brain were in the same cluster as the P region of the virus isolated from the original bovine. Thus, genetic variation in BoDV-1 was extremely low. The phylogenetic analysis revealed that BoDV-1 isolates obtained in this study were part of the same cluster, which suggested that BoDV-1 of the same cluster was widespread among animals in Hokkaido. |
| |
| Keywords: | borna disease genetic variation Japan phylogenetic analysis |
|
|
