| 一种用高拷贝质粒载体制备BAC载体基本功能基因的新方法 |
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| 引用本文： | 崔红玉,王亚萍,徐明举,薛永志,石星明,兰德松,王云峰,童光志.一种用高拷贝质粒载体制备BAC载体基本功能基因的新方法[J].农业科学与技术,2008,9(2):70-75. |
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| 作者姓名： | 崔红玉 王亚萍 徐明举 薛永志 石星明 兰德松 王云峰 童光志 |
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| 作者单位： | [1]河北北方学院动物科技学院动物医学系,河北张家口075131 [2]河北省张家口市宣化区动物检疫站,河北张家口075100 [3]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001 |
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| 摘 要： | 介绍一种改进的用普通高拷贝质粒pUC119来制备细菌人工杂色体（BAC）载体基本功能基因序列的新方法及其在构建分子克隆化病毒中的应用，该方法可使BAC载体基本功能基因序列的制备更为简易、高效。
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| 关 键 词： | 高拷贝质粒载体 制备方法 BAC载体 基本功能基因 细菌人工杂色体 |
Research on the Construction of Bacterial Artificial Chromosome Vector DNA and the Potentials in Application |
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| CUI Hong-yu,WANG Ya-ping,XU Ming-ju,XUE Yong-zhi,SHI Xing-ming,LAN De-song,WANG Yun-feng,TONG Guang-zhi.Research on the Construction of Bacterial Artificial Chromosome Vector DNA and the Potentials in Application[J].Agricultural Science ＆ Technology,2008,9(2):70-75. |
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| Authors: | CUI Hong-yu WANG Ya-ping XU Ming-ju XUE Yong-zhi SHI Xing-ming LAN De-song WANG Yun-feng TONG Guang-zhi |
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| Institution: | CUI Hong-yu, WANG Ya-ping , XU Ming-ju , XUE Yong-zhi , SHI Xing-ming, LAN De-song, WANG Yun-feng, TONG Guang-zhi( 1. Department of Animal Medicine, Hebei North University, Zhangjiakou 075131 ; 2. Animal Quarantine Station of Xuanhua District, Zhangjiakou City, Hebei Province, Zhangjiakou 075131 ; 3. National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001) |
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| Abstract: | Objective] The aim of this study was to provide a method for solving the problems in preparing BAC vector with High-copy plasmid pUC119-Bluelox BAG. Method] With 8electing a proper single restriction site, sequences of a single copy BAC vector plasmid were inserted into proper site of High-copy plasmid pUC119 vector. Result] The gene sequence of BAG vector lost control function of single copy number in new plasmid pUC119-BAC and was copied through High-copy form. The gene sequence of BAC vector basic function was completely cutted off through single enzyme digestion and the control function of single copy could be recovered by auto-connection. Conclusion ] The High-copy pUC119-BAC plasmid was used to copy and amplify high copy of basic function gene sequence in BAG vector, besides thai it could be used to construct transfer vector of molecular cloned recombinant virus or BAC library. |
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| Keywords: | Bacterial artificial chronmsome (BAC) High-copy plasmid BAC vector Single-copy plasmid |
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