发光酶基因标记荧光假单胞菌X16L2的发光研究

通过光光光度仪和ＡＭＰＮ法定量测定荧光假单胞菌Ｘ１６Ｌ２，在液培和土壤中的发光情况及其数量，发现Ｘ１６Ｌ２在液培和土才壤中的发光动力学曲线相似，达到稳定发光强度的时间均在加入反应底物癸醛后约７分钟。Ｘ１６Ｌ２在液培中的发光强度与生物量吴正相关。土壤中Ｘ１６Ｌ２的发光量既与活菌数有关，也与土壤条件有关，土著微生物对Ｘ１６Ｌ２的光量，土壤中Ｘ１６Ｌ２的发光量既与活菌数有关，也与土壤条件有关。土著微生物

BIOLUMINESCENCE OF LUXAB-GENES-MARKED PSEUDOMONAS FLUORESCENS X16L2

A luminescence measurement of the luxAB-genes-marked strain (Pseudomonas fluoreseens X16L2) was carried out in liquid culture and soil microcosms.The curves of luminescence kinetics of X16L2 in the liquid culture and soils were similar.The time reaching stable luminescence was about 7 nun after the luciferase substrate (n-decanal) was added.The results also showed that the bioluminescence could reflect the biomass of X16L2 in liquid culture,and the light output of X16L2 was closely related to not only the number.of viable cells but also the cell physiological activity.Soil conditions and native microorganisms had great effects on the survival of the introduced strain,including the number of viable cells and light output as well as the potential luminescence of X16L2.This study also demonstrated that luminometry could not only in situ detect the physiological activity of lux-genes-marked strain in the samples,but also measure its population indirectly.This method was a rapid and economical technique with high stability and sensitivity,and strong selectivity for tracking and recovering target microorganisms released to the environments.