| 黄瓜枯萎病拮抗菌的拮抗促生因子检测及生物效应研究 |
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| 引用本文： | 马兴,董星晨,张健,罗超越,邓德雷,张春红,王友玲,邱慧珍.黄瓜枯萎病拮抗菌的拮抗促生因子检测及生物效应研究[J].干旱地区农业研究,2018,36(3):130-138. |
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| 作者姓名： | 马兴 董星晨 张健 罗超越 邓德雷 张春红 王友玲 邱慧珍 |
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| 作者单位： | 甘肃农业大学资源与环境学院/甘肃省干旱生境作物学重点实验室 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)(2015CB150501)；国家自然科学基金(31360500)；国家公益性行业(农业)科研专项(201103004)；白银市白银区科技计划项(2014-5N)；甘肃农业大学校创新基金(GAU-CX1021) |
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| 摘 要： | 以黄瓜枯萎病病原菌,尖孢镰刀菌黄瓜专化型(FOC,Fusarium oxysporum f.sp.cucumerinum)为靶标筛选拮抗菌。通过初筛和复筛,获得2株对尖孢镰刀菌黄瓜专化型拮抗效果稳定、拮抗能力较强的细菌QHZ-Yb1和QHZ-Yb2(以下简称,Yb1和Yb2),采用平板对峙法测定其拮抗作用,抑菌带宽度达3.37 mm和3.42 mm,抑菌圈直径2.7 cm,抑菌率分别达到61.9%和62.3%;通过形态学结合生理生化及菌株的16S rDNA序列分析,初步确定这两株菌均为萎缩芽孢杆菌(Bacillus atrophaeus);潜在拮抗促生因子的测定结果表明:菌株Yb1和Yb2具有多种拮抗促生因子,其脂肽类粗提取物的抑菌率分别达到57.4%和58.0%,生长素IAA的分泌量可达1.56 mg·L~(-1)和3.69 mg·L~(-1),溶P量为0.77 mg·L~(-1)和1.19 mg·L~(-1),两个菌株均可产嗜铁素、分泌酪蛋白酶;种子萌发试验结果表明:Yb1和Yb2菌液均可促进黄瓜种子的萌发;菌株生物效应的盆栽试验结果表明:Yb1、Yb2以及Yb1+Yb2菌悬液的处理不仅对黄瓜枯萎病有明显的生防效果,而且可显著增加黄瓜植株的生物量,增加黄瓜植株根际土中细菌和放线菌的数量,减少真菌和尖孢镰刀菌的数量,尤其以Yb1+Yb2菌悬液的处理效果最显著,对黄瓜枯萎病的发病率降低11.11个百分点,植株生物量增加56.7%。结论:菌株Yb1和Yb2具有生防潜力,两菌株配合运用效果更好。
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| 关 键 词： | 尖孢镰刀菌黄瓜专化型 黄瓜枯萎病 拮抗菌 萎缩芽孢杆菌 |
Determining antagonistic and growth promoting factors of bacteria strains against cucumber Fusarium wilt and studies on their biological effects |
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| MA Xing,DONG Xing-chen,ZHANG Jian,LUO chao-yue,DENG De-lei,ZHANG Chun-hong,WANG You-ling,QIU Hui-zhen.Determining antagonistic and growth promoting factors of bacteria strains against cucumber Fusarium wilt and studies on their biological effects[J].Agricultural Research in the Arid Areas,2018,36(3):130-138. |
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| Authors: | MA Xing DONG Xing-chen ZHANG Jian LUO chao-yue DENG De-lei ZHANG Chun-hong WANG You-ling QIU Hui-zhen |
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| Institution: | College of Resources and Environmental Sciences/Gansu Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China,College of Resources and Environmental Sciences/Gansu Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China,College of Resources and Environmental Sciences/Gansu Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China,College of Resources and Environmental Sciences/Gansu Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China,College of Resources and Environmental Sciences/Gansu Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China,College of Resources and Environmental Sciences/Gansu Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China,College of Resources and Environmental Sciences/Gansu Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China and College of Resources and Environmental Sciences/Gansu Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China |
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| Abstract: | |
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| Keywords: | Fusarium oxysporum f sp cucumerinum cucumber Fusarium wilt antagonistic bacteria Bacillus atrophaeus |
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