| 猪瘟与高致病性蓝耳病混合感染的诊断及病原特性分析 |
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| 引用本文： | 杨小燕,刘建奎,戴爱玲,李晓华.猪瘟与高致病性蓝耳病混合感染的诊断及病原特性分析[J].安徽农业科学,2010,38(34):19518-19520. |
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| 作者姓名： | 杨小燕 刘建奎 戴爱玲 李晓华 |
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| 作者单位： | 龙岩学院生命科学学院龙岩学院动物医学研究所福建省人畜寄生与病毒性疫病防控工程技术研究中心,福建省预防兽医学与生物技术高校重点实验室,福建龙岩364000 |
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| 基金项目： | 福建省区域科技重大项目，福建省科技厅创新平台建设项目 |
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| 摘 要： | 目的]诊断由猪瘟病毒（CSFV）、猪繁殖与呼吸综合征病毒（PRRSV）和猪伪狂犬病毒（PRV）引发的猪疫病,并分析病原。方法]采集福建某猪场发病猪的组织器官,提取病毒DNA或RNA,PCR检测病毒感染。采用ELISA方法检测CSFV、PRRSV和PRV的感染情况。结果]测序分析和ELISA结果表明,猪场存在PRRSV、CSFV、PRV3种病毒感染。结论]该次猪病疫情主要是由CSFV和高致病性PRRSV混合感染引起。
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| 关 键 词： | 猪瘟 猪繁殖与呼吸综合征 混合感染 |
Diagnosis of Mixed Infection of Classical Swine Fever Virus and Porcine Reproductive and Respiratory Syndrome Virus and its Pathogenic Characteristics |
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| Institution: | YANG Xiao-yan et al(College of Life Sciences of Longyan University;Institute of Veterinary Medicine of Longyan University;Engineering Research Center for the Prevention and Control of Zoonosis;Key Laboratory of Preventive Veterinary Medicine and Biotechnology,Longyan,Fujian 364000) |
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| Abstract: | Objective] To diagnose swine diseases caused by CSFV,PRRSV and PRV and analyze the pathogenic characteristics.Method]The tissues and viscera of the diseased swine were collected from a hoggery in Fujian Province.DNA and RNA were extracted for PCR amplification and sequencing.ELISA method was used to determine CSFV,PRRSV and PRV infection.Result] The sequencing analysis and ELISA results showed that the mixed infection was caused by CSFV,PRRSV and PRV.Conclusion] The swine epidemic situation was mainly caused by CSFV and PRRSV. |
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| Keywords: | Classical swine fever Porcine reproductive and respiratory syndrome Mixed infection |
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