寒地粳稻外源基因转化途径研究初报

试验研究了寒地粳稻（ＤＳ２＃）成熟胚为受体的ＪＱ７００型基因枪转化，寒地粳稻（ＤＳ３＃）下胚轴愈伤悬浮细胞与细胞团为受体的农杆菌ＬＢＡ４４０４共培养转化，以及寒地粳稻（ＤＳ２ＧＧ＃）未受精胚囊为受体的花粉管通道等转化途径的初步尝试。结果首先在基因枪转化途径上得到突破——成功地将ＮＰＴⅡ基因、ＧＵＳ基因及抗虫基因（Ｂ．ｔ）的ＤＮＡ片段导入寒地粳稻（ＤＳ２＃）成熟胚。以ＧＵＳ基因组织化学法检测外源基因的表达，结果转化水稻细胞的短暂表达为阳性。经过一系列组织培养过程，诱导获得了绿色转基因水稻植株，取其叶片进行外源基因的稳定遗传与表达检测，结果ＧＵＳ基因组织化学检测亦为阳性，Ｏｌｙｍｐｕｓ倒置显微镜镜检结果——转基因水稻（ＤＳ２＃）植株叶片的叶脉等维管组织中ＧＵＳ基因表达更强烈。本文只是对转化途径进行研究，关于Ｂ．ｔ基因的整合与表达的检测工作正在进行中

STUDIES ON METHODS OF FOREIGN GENES TRANSFER INTO RICE ( ORYZA SATIVEA L.)

Studies on three pathways of the foreign gencs transfcrmation: particle bombardment (JQ700 type gene gun) in DS2# ( Oryza Sativa L.), co-transformation of Agrobacterium LBA4404 with DS3# ( Oryza Sativa L.) and pollen tubes passage in embrygenic tissue of DS2#. The results firstly showed on that particle bombardment transfer, vhich foreign DNA such as: neo, gus and cry has been transfered into maturc cmbryo of the ricc (DS2#). Thrcc months latcr, transgenic rice plants were detected by GUS histochemical method. The detected result was the same of the soonly expressing result, which was male reaction. Expecially the cells of vascular bundle in the leave of transgenic rice plants were expressed further more under the Olympus microscope. What we discusscd is only about the mcthod of forcign gcncs transfermation. The dectection of expresstion on cryA gene is operationg in our laberation.