Prepuberal Ovine Mammary Development is Unaffected by Ovariectomy |
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| Institution: | 1. Department of Dairy Science Virginia Polytechnic Institute, Blacksburg, Virginia, USA;2. State University Blacksburg, Blacksburg, Virginia, USA, 24061-0315;3. Dairy Science Group, AgResearch Ruakura, Hamilton, New Zealand.;1. Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4, Canada;2. The Animal Health Center, BC Ministry of Agriculture, 1767 Angus Campbell Road, Abbotsford, British Columbia V3G 2M3, Canada;3. Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta T2N 4N1, Canada;4. Greenbelt Veterinary Services Ltd., 8451 Harvard Place, Chilliwack, British Columbia V2P 7Z5, Canada;5. Molecular Parasitology Section, Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, MD, USA;1. Virology Research Laboratory, Serology and Virology Division (SAViD), Prince of Wales Hospital, Sydney, NSW, Australia;2. School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia;3. Kirby Institute, University of New South Wales, Sydney, NSW, Australia;4. Serology and Virology Division (SAViD), NSW Health Pathology East, Department of Microbiology, Prince of Wales Hospital, Sydney, NSW, Australia;1. Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou 510006, China;2. Institute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai 200031, China;3. College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China;4. School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China;5. Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China;6. International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China;1. A.N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Vavilov Str. 28, 119991 Moscow, Russia;2. Lomonosov Moscow State University of Fine Chemical Technologies, 86 Vernadskogo Pr., 119571 Moscow, Russia;3. Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa St., 93-232 Lodz, Poland;1. Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China;2. School of Environment and Architecture, University of Shanghai for Science and Technology, Shanghai 200093, China;3. Jiangsu Key Laboratory of E-waste Recycling, School of Chemistry and Environmental Engineering, Jiangsu University of Technology, Changzhou 213001, China;1. Department of Medicine, Duke University Medical Center, Durham, NC 27710, United States;2. Duke Cancer Institute, Division of Medical Oncology, Department of Medicine, Duke University Medical Center, DUMC 3841, Durham, NC 27710, United States;3. Durham VA Medical Center, 508 Fulton Street, Durham, NC 27705, United States;4. Division of Medical Oncology, Department of Medicine, Duke University Medical Center, Durham, NC 27710, United States;5. Division of Medical Oncology, Department of Medicine, Duke Cancer Institute, DUMC 103861, Durham, NC 27710, United States |
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| Abstract: | Crossbred ewe lambs were used in a 2 × 2 × 2 factorial design to determine the effect of ovariectomy, age, and estrogen administration on prepuberal mammary development. Intact (I, n = 20) and ovariectomized (OVX, n = 17) lambs were sacrificed at 6 and 13 wk of age after 1 wk of either estrogen 0.1 mg/kg BW/day, s.c.) or vehicle injections. Ovariectomies were performed at 10 ± 1 d of age. At sacrifice, one mammary gland was dissected into parenchymal and stromal fractions for biochemical analyses. Parenchymal explants from the remaining gland were labeled with 3H]thymidine for histoautoradiography. Neither ovariectomy nor estrogen treatment affected body weight, total gland weight, or parenchymal weight (P > 0.05). However, means for each of these variables increased between 6 and 13 wk (P < 0.01). Analysis of relative mammary growth showed significant positive deviations from isometry. However, no significant difference in parenchymal allometry was observed between I and OVX groups. Exogenous estrogen stimulated an increase (P < 0.05) in epithelial cell labeling with 3H]thymidine, but neither ovariectomy nor age at sacrifice had any effect (P > 0.2) on epithelial labeling. These results demonstrate that prepuberal allometric mammary growth in the ewe lamb does not require the presence of the ovary. |
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