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Occurrence of antibodies to Toxoplasma gondii and Lepstospira spp. in manatees (Trichechus inunguis) of the Brazilian Amazon
Authors:Mathews Patrick D  da Silva Vera M F  Rosas Fernando C W  d'Affonseca Neto José A  Lazzarini Stella M  Ribeiro Daniella C  Dubey Jitender P  Vasconcellos Silvio A  Gennari Solange M
Affiliation:Department of Parasitology, Institute of Biomedical Sciences, University of S?o Paulo, S?o Paulo, 05508-900 S?o Paulo, Brazil.
Abstract:The presence of Toxoplasma gondii and Leptospira spp. antibodies was investigated in 74 manatees (Trichechus inunguis [Mammalia: Sirenia]) kept in captivity in two rescue units in the northern region of Brazil. Antibodies to T. gondii were detected in 29 (39.2%) of 74 animals by using the modified agglutination test (titer, 1:25). For antibodies against Leptospira spp., sera were diluted 1:50 and tested against 24 strains ofleptospires by microscopic agglutination microtechnique, and positive samples were end titrated. Twenty-three (31.1%) of 74 animals were reactive to four serovars (Patoc 21/23, Castellonis 2/23, Icterohaemorrhagiae 1/23, and Butembo 1/ 23), with titers ranging from 100 to 1,600. This is the first report of antibodies against T. gondii and Leptospira spp. in T. inunguis from the Brazilian Amazon.
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