| 欧洲大黄的组织培养和快速繁殖 |
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| 引用本文： | 郝道合,贾文庆. 欧洲大黄的组织培养和快速繁殖[J]. 信阳农业高等专科学校学报, 2011, 21(2): 113-115 |
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| 作者姓名： | 郝道合 贾文庆 |
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| 作者单位： | 河南科技学院,园林学院,河南,新乡,453003 |
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| 摘 要： | 以欧洲大黄种子为材料,进行了组织培养和快速繁殖技术的研究。试验结果表明:欧洲大黄种子获取无菌实生苗的较好处理方式是将种子剥壳,胚根垂直向下;种子进行无菌萌发用0.1%的氯化汞溶液消毒时,消毒时间以8分钟为宜;子叶是对愈伤诱导的最佳外植体;茎芽诱导的最佳培养基是MS+BA1.0mg/l+NAA1.0mg/l;1/2MS是适宜的生根培养基。
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| 关 键 词： | 欧洲大黄 组织培养 快速繁殖 |
Tissue culture and rapid propagation of Rheum rhap onticurn L. |
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| HAO Dao-he,JIA Wen-qing. Tissue culture and rapid propagation of Rheum rhap onticurn L.[J]. Journal of Xinyang Agricultural College, 2011, 21(2): 113-115 |
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| Authors: | HAO Dao-he JIA Wen-qing |
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| Affiliation: | HAO Dao-he1,JIA Wen-qing2(Dept.of Garden,Henan Institute of Science and Technology,Xinxiang 453003,China) |
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| Abstract: | With the material of Rheum rhap onticurn L.,tissue culture and rapid reproduction technology are used in this research.The results of this research show that a better way to get a sterile seedling of Rheum rhap onticurn L.is to husk the seed and let the radicle vertically downward;in sterile germinating the seed,it should be disinfected with 0.1% mercuric chloride solution for 8 minutes.Cotyledon is the optimal explant for callus inductions;the optimal culture medium for stem eye induction is MS+BA1.0mg/l+N... |
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| Keywords: | Rheum rhap onticurn L. tissue culture rapid reproduction |
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