16个鱼腥草基因型遗传多样性的SRAP分析

2009年以16个鱼腥草为材料,通过正交设计研究了鱼腥草的SRAP-PCR反应体系和扩增体系,并运用SRAP标记技术构建了16个鱼腥草材料的SRAP-PCR图谱,同时应用DPS分析软件构建了UPGMA聚类图。结果表明:(1)最佳反应体系为总体积25μL中含40ngDNA,0.1mmol/LdNTPs,2.0mmol/LMg2+,37.5ng/μL引物和2UTaq酶。(2)从360对引物中筛选出条带清晰多态性好的118对,共扩增出7582个条带,其中多态性条带6590个,多态率为86.92%。(3)ZY06-028在Me9-GA18扩增产物的250bp处有特异性缺失带,而ZY06-016和ZY06-028在550bp处有特异性缺失带;在Me8-Em10的扩增产物中ZY06-42和ZY06-01分别在300bp和450bp处出现特异性的带,这些带可用来鉴定不同的鱼腥草基因型。(4)在遗传距离0.31处,可将16个鱼腥草基因型分为3个类群,其中ZY06-024和ZY06-01鱼腥草与其它基因型显著不同,分别单独聚为一类,其余的聚为一类。

Genetic Diversity Analysis of 16 Genotypes of Houttuynia cordata Thunb by SRAP

The SRAP amplified and reaction systems were researched and SRAP--PCR map of 16 genotypes of Houttuynia cordata Thunb were constructed and a UPGMA dendrogram was obtained by applying DPS software. The results were as follows： （1） The best reaction system was 40 ng DNA, 0. 1 mmol/L dNTPs, 2. 0 mmol/L Mg2＋, 37. 5 ng/μL primer and 2 U Taq polymerase in 25 μL system. （2） 118 pairs of primers were selected from 360 pairs and 7 582 bands were amplified, of which 6 590 were polymorphic, and the polymorphic percentage of bands was 86. 92%. （3） In amplified products of Meg--GA18, specific deletion band of genotype ZY06-028 was at 250 bp, and which of ZY06-016 and ZY06- 028 were at 550 bp. In amplified products of Me8--Em10, specific deletion band of ZY06-42 and ZY06-01 was at 300 bp and 450 bp respectively. These results can be used to identify genotypes of Houttuynia cordata Thunb. （4） At the genetic distance of 0. 31, 16 genotypes of Houttuynia cordata Thunb could be divided into 3 groups, in which ZY06-024 and ZY06- 01 were significantly different from other types, so they were clustered into one group respectively, and the rest genotypes were clustered into one group.