| 2006年-2008年河南省PRRSV分子流行病学调查 |
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| 引用本文： | 邱骏,张学东,沈志强,赵军,张泉鹏,丁壮.2006年-2008年河南省PRRSV分子流行病学调查[J].内蒙古农业大学学报(自然科学版),2011,32(1):34-40. |
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| 作者姓名： | 邱骏 张学东 沈志强 赵军 张泉鹏 丁壮 |
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| 作者单位： | 1. 吉林大学畜牧兽医学院,长春,130062
2. 山东省滨州畜牧兽医研究院,滨州,256600 |
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| 基金项目： | 广东省科技攻关重大项目(2008A020100020) |
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| 摘 要： | 根据GenBank公布的猪繁殖与呼吸综合征病毒(PRRSV)ATCC VR-2332株的NSP2,ORF5-7基因的核苷酸序列,设计合成5对特异性引物,用RT-PCR方法扩增出10株PRRSV河南地方株的NSP2和ORF5-7片段,将扩增片段克隆到pMD-18T载体并进行测序。应用DNAStar序列分析软件对分离株的NSP2和ORF5-7基因和其推导的氨基酸序列与不同来源的PRRS毒株进行了同源性比较,结果表明PRRSV河南分离株的NSP2和ORF5-7基因与不同来源美洲型毒株之间的同源性分别为89.1%-98.1%和81.4%-96.1%,而与欧洲型毒株之间的同源性仅为46.7%-61.7%和44.7%-45.9%;同时将PRRSV河南分离株NSP2和ORF5-7基因的推导氨基酸序列与其他美洲型PRRSV毒株进行变异分析比较,证实了PRRSV河南分离株NSP2和ORF5-7基因发生了变异。
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| 关 键 词： | PRRSV NSP2基因 ORF5-7基因 变异分析 |
MOLECULAR EPIDEMIOLOGY OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS IN HENAN PROVINCE FROM 2006-2008 |
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| QIU Jun,ZHANG Xue-dong,SHEN Zhi-qiang,ZHAO Jun,ZHANG Quan-peng,DING Zhuang.MOLECULAR EPIDEMIOLOGY OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS IN HENAN PROVINCE FROM 2006-2008[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),2011,32(1):34-40. |
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| Authors: | QIU Jun ZHANG Xue-dong SHEN Zhi-qiang ZHAO Jun ZHANG Quan-peng DING Zhuang |
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| Institution: | QIU Jun1,ZHANG Xue-dong1,SHEN Zhi-qiang2,ZHAO Jun1ZHANG Quan-peng1,DING Zhuang1 (1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China,2.Shandong Binzhou Animal Science and Veterinary Medicine Academy,Binzhou 256600,China) |
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| Abstract: | Five pairs of specific primer of Nsp2 and ORF5-7 genes were designed according to the gene sequence of ATCC VR-2322 of American standard strain.The cDNA fragment of PRRSV Henan strains were amplied by RT-PCR,then cloned into pMD-18T vector respectively and sequenced.After sequencing,the Nsp2 and ORF5-7 genes were blasted with PRRSV genome sequence downloaded from NCBI.Compared with other American isolated,the homology rate of the Nsp2 and ORF5-7 genes and amino acid were 89.1% to 98.1% and 81.4% to 97.1% re... |
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| Keywords: | PRRSV Nsp2 gene ORF5-7 gene variation analysis |
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