| 变叶海棠遗传多样性的AFLP分析 |
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| 引用本文： | 石胜友,成明昊,梁国鲁,郭启高,李晓林,周志钦. 变叶海棠遗传多样性的AFLP分析[J]. 园艺学报, 2006, 33(2): 381-384 |
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| 作者姓名： | 石胜友 成明昊 梁国鲁 郭启高 李晓林 周志钦 |
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| 作者单位： | (湖北省宜昌市林业局, 宜昌443000; 西南大学园艺园林学院, 重庆市果树学重点实验室, 重庆400716) |
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| 摘 要： | 用8对引物对变叶海棠30个不同的变异类型进行了AFLP分析, 共扩增出656条带, 其中多态性带为568条, 平均每对引物扩增出71条多态性带, 多态性带百分率为86.59% , 各变异类型多态性比例介于0.3582～0.6296之间; 依据简单匹配系数对AFLP扩增结果进行UPGMA聚类, 30个变异类型在0.68水平上分为12个类群; 该试验从DNA水平上探明了变叶海棠的遗传多样性是变叶海棠与陇东海棠和花叶海棠产生渗入杂交形成的。
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| 关 键 词： | 变叶海棠 AFLP 遗传多样性 聚类分析 |
| 文章编号： | 0513-353X（2006）02-0381-04 |
| 收稿时间： | 2005-04-18 |
| 修稿时间： | 2005-04-182005-08-22 |
Genetic Diversity of Malus toringoides (Rehd.) Hughes Based on AFLP |
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| Shi Shengyou,Cheng Minghao,Liang Guolu,Guo Qigao,Li Xiaolin,Zhou Zhiqin. Genetic Diversity of Malus toringoides (Rehd.) Hughes Based on AFLP[J]. Acta Horticulturae Sinica, 2006, 33(2): 381-384 |
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| Authors: | Shi Shengyou Cheng Minghao Liang Guolu Guo Qigao Li Xiaolin Zhou Zhiqin |
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| Affiliation: | ( State Forestry Administration of Yichang, Yichang 443000, China; College of Horticulture and Landscape, Southwest University, the Key Laboratory for Pomology of Chongqing, Chongqing 400716, China) |
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| Abstract: | The study is mainly on the genetic diversity of Malus toringoides(Rehd.) Hughes by applying AFLP. 658 bands were obtained with 8 pairs of AFLP primers based on 30 variant types, and among them 568(86.59%) were polymorphic with an average of 71 bands for per AFLP primer, the ratio of polymorphic rate varied from 0.3582 to 0.6296. UPGMA cluster analysis based on simple matching coefficient(SM)divided the thirty variant types into twelve groups at 0.68 level. At DNA level, the results showed that the rich diversity in M.toringoides(Rehd.) Hughes was derived from the introgression of M.toringoides(Rehd.)Hughes with M. transitoria Schneid. and M. kansuensis Rehd. |
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| Keywords: | AFLP |
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