| 黄海棠的组织培养和快速繁殖 |
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| 引用本文： | 雷颖.黄海棠的组织培养和快速繁殖[J].甘肃农业大学学报,2003,38(3):357-360. |
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| 作者姓名： | 雷颖 |
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| 作者单位： | 甘肃林业职业技术学院园林系,甘肃,天水,741020 |
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| 摘 要： | 以黄海棠茎切段为外植体,进行植株再生和快速繁殖研究。结果表明在MS+BA 3 mg/L+NAA 0.4 mg/L培养基上,不定芽诱导效果较好,分化率达100 %;增殖培养时,在MS+BA 2 mg/L+IAA 0.2 mg/L+GA3 0.3 mg/L的培养基上增殖的嫩茎转入MS+BA 1 mg/L+IAA 0.2 mg/L+ GA3 0.3 mg/L的培养基上增值倍数降低,但芽苗增高增粗;再生苗在1/2 MS+IAA(0.1～0.5)mg/L的培养基上生根效果较好。
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| 关 键 词： | 黄海棠 组织培养 快速繁殖 |
| 文章编号： | 1003-4315(2003)03-0357-04 |
Tissue culture and rapid propagation ofHyperlcum ascyron |
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| LEI Ying.Tissue culture and rapid propagation ofHyperlcum ascyron[J].Journal of Gansu Agricultural University,2003,38(3):357-360. |
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| Authors: | LEI Ying |
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| Abstract: | Using stem cuttings of Hyperlcum ascyron as callus for the study on fast propagation. The results indicates: MS+BA 3 mg/L+NAA 0.4 mg/L is the best medium, by which the induction effect is better and the differentation rate reaches to 100 %. The seedling has a remarkable increase in height and diameter when is transferred from MS+BA 2 mg/L+IAA 0.2 mg/L+GA3 0.3 mg/L to MS+BA 1 mg/L+IAA 0.2 mg/L+GA3 0.3 mg/L. The result also shows that the 1/2 MS+IAA (0.1～0.5) mg/L is suitable for striking roots. |
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| Keywords: | Hyperlcum ascyron tissue culture rapid propagation |
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