有关蚕豆根尖微核试验的问题分析及改进策略

蚕豆根尖微核技术是1种新兴的快速生物测试方法。该方法已被列入国家＂生物监测技术规范（水环境部分）＂,而且在监测重金属、有机污染物等环境致突变性物质方面也得到了充分应用。综述了蚕豆根尖细胞微核技术从产生到最终确立的进程,并重点就实际操作过程中存在的一些问题进行了讨论,提出了相应的改进策略,以期为蚕豆根尖微核试验技术的完善和广泛使用提供依据。

Problems of Micronucleus Test in Vicia faba Root Tips and Improvement Strategy

The micronucleus technology in Vicia faba root tips was a new rapid bioassay method,which was arrayed in the National Biological Monitoring Technical Specification for Water Environment.It was widely used in monitoring the heavy metals,organic pollutants and other environmental mutagenic matters.The development process of micronucleus technology in V.faba root tips was introduced,the problems in practical operation were discussed,and the improvement strategies were put forward to provide the basis for the perfection and wide application of the technology.