小滨麦易位系山农6343抗白粉病基因的分子标记定位

本研究通过抗性接种鉴定对从普通小麦品种烟农15与八倍体小滨麦杂交后代中选育的抗白粉病小滨麦易位系山农6343的白粉病抗性遗传特点进行了分析,结果表明,山农6343的白粉病抗性由显性单基因控制,暂将其命名为PmSn6343(t);利用辉县红与山农6343杂交构建了包含302个家系的F2分离群体,对其进行白粉病抗性基因分子标记的连锁分析和染色体定位。在分析的1980对SSR、EST-SSR和STS引物中,有403对基因组SSR引物可在亲本间揭示多态性差异,其中Wmc658和Barc122两个引物在优选小群体中可以扩增出多态性谱带;采用F2群体对两个标记进行连锁分析证明Wmc658和Barc122与山农6343抗白粉病基因PmSn6343(t)的连锁距离分别为3.4cM和5.4cM,并将抗白粉病基因定位在染色体2AL上。利用F2:3家系对两个标记进行验证,结果表明,两个标记是与白粉病抗性基因PmSn6343(t)连锁的可靠分子标记。

Powdery Mildew Resistant Locus in Tritileymus Translocation Line Shannong6343 Mapped by Molecular Markers

In present studies,we analyzed the genetic characteristics of powdery mildew resistant locus in Tritileymus Translocation line Shannong6343 that was derived from the offspring of a cross between the common wheat cultivar Yannong15 and octoploid Tritileymus,the results demonstrated that the powdery mildew resistant locus in Shannong6343 was a single dominant locus based on the phenotypic data from artificial inoculation,tentative named the locus as PmSn6343(t).In order to figure out the linkage relationship and chromosome location of powdery mildew resistant locus in Shannong6343,an F2 segregating population with 302 individuals derived from the cross between Huixianhong and Shannong6343 was employed to be genotyped by the molecular markers of SSR,EST-SSR and STS.403 pairs of SSR markers from the tested 1 980 markers of SSR,EST-SSR and EST were identified to be polymorphisms between the two parents,of which two SSR markers,Wmc658 and Barc122,can generate polymorphisms in the Preferred Small Group(PSG),and then two markers were employed to phenotype the 302 F2 individuals.The results indicated that the genetic distances between PmSn6343(t) and Wmc658 or Barc122 were 3.4 cM and 5.4 cM,respectively,and PmSn6343(t) could be assigned to chromosome 2AL,which was validated by progeny test of F2:3 families.In conclusion,we thought that two identified markers should be reliable linked to the locus PmSn6343(t).