辣椒DH群体果实性状的分离及与F2群体的比较

为分析DH群体的遗传稳定性及其DH群体内性状分离与同来源的F2群体之间异同,本研究利用辣椒花药培养技术,构建一个由“羊角椒(97403)×方灯笼甜椒(97410)”的杂交而成的牛角椒组合作为供体的且由103个DH系组成的DH群体,对该DH群体进行单果重等5个主要果实性状的遗传表现进行分析,并与同来源的F2群体进行比较。结果表明:原供体及其双亲的5个果实性状的变异系数均较小,三者之间的各性状均存在显著差异,说明原供体及其双亲的整齐一致性;对DH群体和F2群体各性状的平均值、变异系数及其性状分离区间的分析和对比结果表明,5个果实性状均是受多基因控制的数量性状,且由于基因重组无论是DH群体还是F2群体均能产生正向和负向两个方向的超亲基因型。但DH群体与F2群体比较各性状的分离区间明显增大,超亲分离类型明显增加,且获得的各DH系均为稳定遗传的自交系,提高了花培育种的选择效率。

The Separation of The Fruit Traits in The DH Population of Capsicum annuum L and the Comparison with DH Population and F2 Population

In order to analyze the stability of inheritance about the DH population and explore the difference of the segregations of fruit traits between the DH population and the F2 population, One DH population of pepper was constructed by utilizing the anther culture technology. This population was consisted of 103 DH lines from the donor pepper with the bull-horn shape, which was hybridized by pepper with the shape of sheep-horn (97403) and sweet pepper with the shape of blocky (97410). Mainly based on 5 fruit traits, such as weight per fruit, the genetic phenotypes of this DH population were analyzed and compared with the homologous F2 population. The coefficient of variation (CV) in 5 fruits traits of the primary donor and its parents was relatively low. Moreover, significant differences were observed in every trait among three populations. These results indicated the uniformity of primary donor and its parents. The average, CV and separated region (SR) were also analyzed and compared in each trait from DH and F2 population. The result suggested that 5 fruit traits were quantitative traits controlled by the multiple genes. Due to gene recombination, both super-parent genotypes with positive and negative aspects were observed in either DH or F2 populations. However, both SR of each trait and super-parent separated types in the DH populations were significantly increased compared with the F2 populations. The obtained DH lines which were all stable-genetic inbreeding lines improved the selection efficiency of anther culture breeding.