枸树组织培养的研究

对枸树的初代培养、继代培养和生根培养进行了研究。初代培养用腋芽、顶芽作为外植体,在灭菌前用半胱氨酸处理10min,以防止褐变。培养出以芽丛为主,以茎段为辅的无性繁殖系。其培养基为MS BA1.0mg/L NAA0.1mg/L。继代培养时采用芽丛和茎段进行增殖,其培养基为MS BA0.5mg/L。在培养基中增加蔗糖的用量和降低光照,可以减轻玻璃化现象。继代苗可以生根,其培养基为1/2MS IBA0.5mg/L,但生根的速度较慢。

The Study of Tissue Culture in Paper Mulberry

The study of Paper mulberry is about its primary culture, secondary culture and rooting culture. The axillary and top buds are used as explants in primary culture, They are treated by ban guang an suang ahead of ten min, so it can prevent brown change and educate out bud clster for main. The optimum medium was MS BA1.0mg/L NAA0.1mg/L. Axillary and single-bud cegments were multiplied in secondary culture . The optimum medium was MS BA0.5mg/L. It gains cane suger to make shine lower and alleviate alss variety phenomenon secondary seedling can take root but specd is slow, The optimum medium was 1/2MS IBA0.5mg/L.