红花长寿花愈伤组织的诱导和植株再生研究

试验采用红花长寿花茎段和叶片作为外植体,在附加不同植物激素组合的培养基中对愈伤组织的诱导和植株再生进行研究。结果表明:不带叶柄的叶片在MS 2,4-D1.0mg/L BA0.5mg/L的培养基上对愈伤组织的诱导和增殖效果最好;在MS BA0.25mg/L 2,4-D1.5mg/L GA0.6mg/L的培养基上有利于愈伤组织转绿分化;在MS NAA0.25mg/L BA1.5mg/L GA0.6mg/L的培养基上有利于丛生芽诱导和增殖;在1/2MS NAA0.3mg/L IBA0.7mg/L培养基中生根效果最好。

Callus induction and Plant Regeneration in Kalanchoe Blossfeldiana with Red Flowers

The stem sections and the leaves from the plants were used as explants and the callus induction and plant regeneration were conducted on the media supplemented with different plant hormone combinations in Kalanchoe blossfeldiana with red flowers in this experiment. The obtained results showed that the MS medium supplemented with 1.0 mg/L 2,4-D and 0.5 mg/L BA was optimal for the callus induction and propagation from leaves without leafstalks; the MS medium supplemented with 0.25 mg/L BA and 1.5 mg/L 2,4-D and 0.6 mg/L GA was beneficial to the differentiation of green callus; and the MS medium supplemented with 1.5 mg/L BA and 0.25 mg/L NAA and 0.6mg/L GA was propitious to the induction and the propagation of bud clusters; and the highest percentage of rooting was obtained on the 1/2 MS medium adding 0.3 mg/L NAA and 0.7 mg/L IBA.