烤烟烟叶腺毛分泌物提取方法初探

为探索烤烟烟叶腺毛分泌物提取效果最佳的方法,以烤烟品种K326、云烟85和红花大金元为材料,采用二氯甲烷作为溶剂,比较不同提取级数和不同提取时间前后叶片重量的变化。结果表明,在烟叶腺毛分泌物提取方法的不同提取级数的效果比较中,3个烤烟品种均以前3级提取的变化最明显,而之后增加提取级数,叶片重量变化不明显;不同提取时间对叶片的重量变化影响不大。这说明采用3级提取作为烤烟叶片腺毛分泌物的提取方法比较适宜。

Study on the Extraction Method of Leaf Trichome Exudates in Flue-cured Tobacco

In order to investigate the best extraction methods of leaf trichome exudates in flue-cured tobacco, three flued-cured tobacco varieties including "K326", "Yunyan85" and "honghuadajinyuan", were used in this study. Effects of different extraction degrees and different lengths of extraction time on leaf weight changes were compared. The results showed that the significant change of weight was found before the third extraction in three flue-cured tobacco varieties, while it''s no significant different in leaf weight change following the increases in extraction degrees. The effect of different extraction degrees on leaf weight change seemed little. This indicates that the three degrees extraction method of leaf trichome exudates in flue-cured tobacco is more effective, which will be useful in study on leaf trichome exudates in flue-cured tobacco for the future.