| 农杆菌介导法转化大豆体细胞胚获得转基因植株 |
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| 作者姓名： | 王晓春 王 罡 王 萍 季 静 刘尚前 |
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| 作者单位： | 天津大学农业与生物工程学院，河北北方学院南校区农业科学系 河北宣化075131，天津大学农业与生物工程学院，淮海工学院海洋学院，天津大学农业与生物工程学院，河北北方学院南校区农业科学系，天津300072，天津300072，连云港222005，天津300072，河北宣化075131 |
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| 基金项目： | 国家科技部“国家植物转基因技术研究开发与中试基地建设”专项课题(J99-B-001)资助。 |
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| 摘 要： | 以六个品种大豆体细胞胚为受体,以Bt基因为目的基因,应用根癌农杆菌介导法对大豆进行了遗传转化,经卡那霉素选择、诱导体细胞胚萌发及壮苗培养,获得了完整的抗性再生苗,经PCR、PCR-Southern和点杂交分析鉴定,初步证明外源基因Bt已整合到大豆的基因组中。
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| 关 键 词： | 大豆 体细胞胚 Bt基因 遗传转化 |
Genetic Transformation of somatic embryos viaAgrobacterium tumefaciens in Soybean |
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| Authors: | Wang Xiaochun Wang Gang Wang Ping Ji Jing Liu Shangqian |
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| Institution: | 1Tian Jin University of Agricultural and Biological engineering Tian jin 300072; 2Department of agriculture sciences of Hebei North University, Xuan Hua 075131; 3Huahai Institute of Technology, Marine School,Lianyungang 222005 |
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| Abstract: | Somatic embryos of 6 different soybean were transformed by Agrobacterium tumefaciens , The resistant plants to kanamycin were obtained. 5 positive plants were obtained among them by PCR , These positive plantlets were further analyzed by dot blotting and PCR-Southern blotting. These results demonstrated that the target gene had been transformed into the soybean genome. |
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| Keywords: | Soybean Somatic embryos Bt gene Genetic transformation |
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