奶牛温氏附红细胞体抗原特性研究

研究目的为制备E.wenyoni特异性诊断抗原及研制高效附红细胞体疫苗提供基础进行试验;方法从自然感染温氏附红细胞体(E.wenyoni)的牛血液中纯化附红细胞体后,采用SDS-PAGE和免疫印迹技术分析E.wenyoni抗原蛋白组分;结果SDS-PAGE结果显示,E.wenyoni全蛋白分子量范围为24kD￣150kD,主要的9种蛋白质分子量分别为141.34kD,107.27kD,86.03kD,68.05kD,56.21kD,44.30kD,32.99kD,28.89kD,24.59kD;免疫印迹筛选出3特异性蛋白质,分子量分别为147.31kD,49.03kD,35.72kD;结论E.wenyoni主要由九种类抗原蛋白组成,3种特异性蛋白质,可作为E.weny-oni特异性诊断抗原及附红细胞体疫苗的保护性抗原。

Study on the Antigenic Characteristics of E.wenyoni in Cow

OBJECTIVEThe study were progressed in order to pave the way for developing a specific-antigen-based assay and good quality vaccine for the E wenyoni control;METHODThe antigenic composition was identified tentatively with SDS-PAGE and western blotting after E.wenyoni was isolated from blood of cattle with E.wenyoni;RESULTSThe result of SDS-PAGE showed that the molecular weights of E.wenyoni antigen covered 24kD~150kD. Nine domain proteins were clearly isolated, and their molecular weights were 141.34 kD, 107.27 kD, 86.03 kD, 68.05 kD, 56.21 kD, 44.30 kD, 32.99 kD, 28.89 kD, and 24.59kD, respectively. Three kinds of immunogenic protein were screened with western blotting, and their molecular weights were 147.31 kD, 49.03 kD and 35.72 kD, respectively;CONCLUSIONE.wenyoni contains nine kinds of domain proteins and three kinds of immunogenic protein. They can be used for specific-antigens-based assay and protective antigens of good quality E wenyoni vaccine.