紫萁孢子繁殖快速成苗技术研究

为了筛选出最佳的紫萁孢子育苗基质,弄清紫萁孢子育苗所需的环境条件,利用在湖北民族学院和杭州城郊两地采集的紫萁孢子,以壤土、山林腐殖土、红砂壤为试验基质进行紫萁孢子遮荫条件下育苗研究。结果表明:在保湿、控制温度、覆盖遮光率为75%的遮阳网的条件下孢子发芽率高,能正常形成原叶体,继续培养均能进入有性世代形成孢子体幼苗,其中在山林腐殖土和壤土按1:1的比例混合的基质上最适于孢子萌发和幼苗生长,出苗量可达3500株/m2;在杭州采集的紫萁孢子萌发后形成的原叶体、孢子体均比在湖北民族学院采集的耐高温性好。

Study on Spore Fast Propagation Seedling in Osmunda japonica Thund.

Screening the best media of spore propagation in Osmunda japonica Thund. and understanding fully that spore propagation in Osmunda japonica Thund. required for the environmental factors, we maked use of Osmunda japonica Thund. spores in Hubei Institute for Nationalities and in the city suburb of Hangzhou to process propagative test in forest soil, farm land soil, red sand soil media by overshadowing. The result indicates that spore germination shapes prothallium at keeping wet, cortrolling temperature and covering with 75% shading rate. If continuously culture, all can enter the sexual generation and shape sporophytic young seedling. Under the aspact of the propagating young seedling, the experiment shows forest humic soil: field soil=1: 1(Volume rate) is controlled most easily among these media. The seedlings in unit area can amount to 3500 plant/m2. Compared with collecting in Hubei Institute for Nationalities, shaded prothallium and sporophyte after collectingOsmunda japonica Thund. spores germinate in Hangzhou both have better heat-tolerance.