燕麦AsRBP1基因克隆及表达特性分析

为了给AsRBP1基因在小麦抗逆转基因分子育种中的应用奠定理论基础,采用同源克隆方法从燕麦中克隆、获得AsRBP1基因,其cDNA序列全长447 bp,编码148个氨基酸.AsRBP1在氨基端具有典型的RNA识别基序(RNA-Recognition Motif,RRM),在羧基端富含甘氨酸,其含量达35.8％.系统进化树分析表明,AsRBP1与拟南芥AtGR-RBP7亲缘关系很近.实时荧光定量聚合酶链式反应(Real-time PolymeraseChain Reaction,RT-PCR)分析该基因的表达特性表明,AsRBP1明显受到外源脱落酸(Abscisic acid,ABA)与低温的诱导,同时对干旱和高盐胁迫也做出响应.由此,推测该基因属于GR-RBPs基因家族成员,可能参与逆境胁迫应答,在增强植物的抗逆性中发挥着重要的作用,可以为小麦抗逆分子育种提供优良候选抗逆基因资源.

Cloning of AsRBP1 Gene from Oat (Avena sterilis) and Analysis on Its Expressions to Stresses

Glycine rich RNA binding proteins (GR RBPs) play important roles in the regulation of stress and improve the resistance in plants. In this study, in order to mine stress related genes and supply important candidate genes for transgenic wheat, AsRBP1 was cloned from Avena sterilis based on homologous cloning method. The results showed that the cDNA of AsRBP1 was 447 bp in length and encoded 148 amino acids. There was a typical RNA recognition motif (RRM) in the N terminal in AsRBP1 and Glycine rich domain in the C terminal, which content was 35.8 %. The Phylogenetic tree showed that AsRBP1 and Arabidopsis AtGR RBP7 had a close relationship. Real time PCR analysis of the expression characteristics showed that AsRBP1 was significantly induced by exogenous abscisic acid and low temperature, and at the same time it responded to drought and high salt stress. Therefore, it was speculated that AsRBP1 was a member of GR RBPs family and might be involved in stress response and enhanced the stress resistance of plants. Finally, it was very useful for wheat molecular breeding aspect to provide good candidate stress genes.