甘蓝型油菜不同抗倒性材料中木质素代谢途径关键基因表达特点

植物体内木质素的含量不仅与植物的抗病性有关，也与植物的抗倒伏性状密切联系。本试验以15个抗倒性等级不同的甘蓝型油菜品种为材料，分别在初花期和青荚期测定茎秆中部木质素含量，同时用qRT-PCR的方法分析了木质素代谢途径中6个关键基因PAL、4CL、C4H、CCR1、CCR2和CAD的表达量差异。结果表明，油菜茎秆木质素从初花期到青荚期平均增加了28%，抗倒性强的材料木质素增加最明显，达到33.5%；不同时期，不同抗倒伏性的油菜茎秆中部木质素含量差异均极显著；各基因表达量在两个时期间差异均显著或极显著，但在不同抗倒性材料间，只有基因PAL、4CL和CCR1表达量间差异显著；基因PAL的表达量与木质素含量相关性最强，4CL基因的表达量与其他关键酶基因表达量相关性最强。综上所述，木质素的合成积累由代谢途径中各个环节关键基因所共同决定，但基因PAL和4CL对木质素的代谢影响更明显。

Expression Characteristics of Key Genes in Lignin Pathway among Different Lodging Resistance Lines of Brassica napus L.

Lignin is related to not only plant disease resistance but also lodging resistance. The lignin content and six key genes (PAL, 4CL, C4H, CCR1, CCR2, and CAD) expression characteristics of the middle stem were analysed at early flowering stage and podding stage of 15 Brassica napus L. varieties with different lodging resistance level. The results indicated that the lignin content generally increased by 28% from early flowering stage to podding stage, especially by 33.5% in the varieties with stronger lodging resistance. Lignin content was significantly different between the varieties with different lodging resistance levels. The expression of key enzyme genes was remarkably different between two stages, and the expression of genes PAL, 4CL, and CCR1 was remarkably different between the varieties with different lodging resistance levels. The expression of gene PAL was significantly correlated with lignin content at both stages, and the expression of gene 4CL was significantly correlated with that of most other genes. Our results suggested that all the genes studied in the paper could control the lignin synthesis, and PAL and 4CL were the most important genes in lignin pathway.