| 甘薯NBS-LRR类抗病基因同源序列的克隆、分析及数目研究 |
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| 引用本文： | 屈满义,查向东,王钰,杨金环,蒋琳,阮龙. 甘薯NBS-LRR类抗病基因同源序列的克隆、分析及数目研究[J]. 热带作物学报, 2008, 29(5): 610-617 |
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| 作者姓名： | 屈满义 查向东 王钰 杨金环 蒋琳 阮龙 |
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| 作者单位： | 安徽大学生命科学学院;安徽省生态工程与生物技术重点实验室,合肥230039;安徽省农业科学院,合肥,230031 |
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| 基金项目： | 安徽省自然科学基金项目 ， 安徽省高校省级自然科学研究重点项目 ， 安徽大学分子药理学创新团队项目 |
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| 摘 要： | 根据植物抗病基因保守序列设计特异引物,对甘薯高抗茎线虫病品种AB94078-1、感病品种徐18及其8个后代品系进行PCR扩增.获得15个具有完整氨基酸读码框的片段,均具有NBS类抗病蛋白4个保守基序,其中5个片段含LRR结构.利用从亲本AB94078-1扩增的R510-AB作为探针进行Southern杂交,结果表明,其在基因组中存在多个拷贝,并估计其在基因组中的拷贝数目,克隆新的甘薯RGA序列,为进一步获得甘薯抗病基因打下基础.
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| 关 键 词： | 甘薯 抗病基因同源序列 NBS-LRR Southern杂交 |
Cloning and Copy Number Estimation of NBS-LRR Based Disease Resistance Gene Analogs from Ipomoea batatas |
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| Qu Manyi,Zha Xiangdong,Wang Yu,Yang Jinhuan,Jiang Ling and Ruan Long. Cloning and Copy Number Estimation of NBS-LRR Based Disease Resistance Gene Analogs from Ipomoea batatas[J]. Chinese Journal of Tropical Crops, 2008, 29(5): 610-617 |
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| Authors: | Qu Manyi Zha Xiangdong Wang Yu Yang Jinhuan Jiang Ling Ruan Long |
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| Affiliation: | 1.School of Life Science, Anhui University;2.Anhui Key Laboratory for Eco-engineering and Biotechnology;1.School of Life Science, Anhui University;2.Anhui Key Laboratory for Eco-engineering and Biotechnology;1.School of Life Science, Anhui University;2.Anhui Key Laboratory for Eco-engineering and Biotechnology;1.School of Life Science, Anhui University;2.Anhui Key Laboratory for Eco-engineering and Biotechnology;1.School of Life Science, Anhui University;2.Anhui Key Laboratory for Eco-engineering and Biotechnology;Anhui Academy of Agricultural Sciences |
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| Abstract: | PCR primers were designed according to the conserved NBS-LRR domains of the disease resistance genes cloned in plants of sweet potato(Ipomoea batatas), and the genomic DNA extracted from Sweet potatoes AB94078-1, Xushu18 and their 8 progenies were utilized as templates. Fifteen resistance gene analogs(RGA)were obtained, which contain 4 motifs of the NBS domain, and 5 of the analogs also possess LRR domain. Southern blotting analysis, using R510-AB as probe, showed that there were multi-copies in the genomic DNA of AB94078-1. The origin and organization of NBS-LRR based disease resistance gene analogs in the Ipomoea batatas genome were studied. The knowledge about these RGAs can further be used for cloning of full-length disease resistance gene in Ipomoea batata genome. |
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| Keywords: | NBS-LRR |
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