短梗胡枝子的组织培养研究（摘要）

目的]研究短梗胡枝子（Lespedeza cyrtobotrya）的组织培养。方法]以短梗胡枝子种子为材料,对其组织培养进行了系统研究。结果]2.1%次氯酸钠灭菌8min,发芽率较高,且污染率为0;在初代培养中,基础培养基为MS,6-BA对分化系数影响达到显著水平,适宜的增殖培养基为MS＋1.00mg/L6-BA＋0.10mg/LNAA＋0.01mg/L2,4-D,分化系数可达6.69;在继代培养中,适宜的培养基为MS＋1.00mg/L6-BA＋0.05mg/L2,4-D;在生根培养中,IBA浓度为0.50mg/L时,生根率和平均生根数都最佳。结论]为进一步开展胡枝子基因工程遗传改良提供了理论依据。

Tissue Culture of Lespedeza cyrtobotrya

Objective] The aim was to carry out study on tissue culture of Lespedeza cyrtobotrya. Method] The seeds of L. cyrtobotrya were used as materials to study on its tissue culture. Result] The best sterilization time to L. cyrtobotrya seeds was 8 min with 2.1% NaClO,in which shooting percent reached 37.8% and no polluted situations occurred. In the primary culture with the MS as basal medium,the concentration of 6-BA showed a significant effect on the index of buds differentiation,the optimum differentiation culture medium was MS+BA 1.0 mg/L+NAA 0.1 mg/L+2,4-D 0.01 mg/L,on which the index of generation could reach 6.69. The optimum subculture medium was MS+6-BA 1.0 mg/L+2,4-D 0.05 mg/L. The plants can generate the highest roots and rooting percent with IBA 0.50 mg/L. Conclusion] This study had provided theoretical basis for genetic improvement of L. cyrtobotrya.