| Mu转座子介导的玉米插入突变体的鉴定 |
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| 引用本文： | 陈果,李见坤,王国英,郑军.Mu转座子介导的玉米插入突变体的鉴定[J].分子植物育种,2011(5):572-578. |
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| 作者姓名： | 陈果 李见坤 王国英 郑军 |
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| 作者单位： | 中国农业科学院作物科学研究所农作物基因资源与基因改良国家重大科学工程 |
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| 基金项目： | 转基因生物新品种培育重大专项(2009ZX08009-019B)资助 |
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| 摘 要： | 选取5个对干旱胁迫响应的玉米蛋白磷酸酶基因,从Mu突变体库中定向筛选到这些基因共92个Mu转座子插入的候选突变体.在田间种植这些候选突变体的后代,每个12粒.利用巢式PCR对这些后代植株的目标插入位点进行鉴定,选择阳性植株与B73杂交或回交.在92个候选突变体中,有21个为可遗传的Mu转座子插入突变体;其中8个突变体的...
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| 关 键 词： | 玉米 Mu转座子 蛋白磷酸酶 巢式PCR 突变体 |
Identification of the Mutator Insertional Mutants in Maize |
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| n Guo,i Jiankun,g Guoying,heng Jun.Identification of the Mutator Insertional Mutants in Maize[J].Molecular Plant Breeding,2011(5):572-578. |
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| Authors: | n Guo i Jiankun g Guoying heng Jun |
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| Institution: | * Institute of Crop Sciences, National Key Facility of Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, 100081 |
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| Abstract: | Ninty-two Mu-insertional candidate mutants of 5 protein phosphatase genes responsive to drought stress were screend from a maize Mu mutant library. Twelve progeny of every candidate mutant were grown and analyzed by nested PCR. The positive plants with Mu-insertion in the target gene were self-pollinated and back crossed with B73 in the field. The results showed that the Mu-insertion of 21 mutants were heritable and the flanking sequences of Mu-insertion were sequenced. Among the 21 heritable mutants, there were 8 insertions in the 5’-UTR, 10 in the introns and 3 in the exons. After several rounds of backcross and selfing, the homozygous Mu-Insertion lines were identified and they will be used for further functional analysis. |
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| Keywords: | Maize (Zea Mays L ) Mutator transposon Protein phosphatase Nest PCR Mutant |
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