| 大白菜永久高密度分子遗传图谱的构建 |
| |
| 引用本文： | 张立阳,张凤兰,王美,刘秀村,赵岫云,薛林宝.大白菜永久高密度分子遗传图谱的构建[J].园艺学报,2005,32(2):249-255. |
| |
| 作者姓名： | 张立阳 张凤兰 王美 刘秀村 赵岫云 薛林宝 |
| |
| 作者单位： | ( 北京市农林科学院蔬菜研究中心, 北京100089; 扬州大学农学院园艺系, 扬州225009) |
| |
| 基金项目： | 北京市科技新星项目(954812900)，国家‘863’项目(2001AA241124,2002AA207012) |
| |
| 摘 要： | 以大白菜高抗TuMV白心株系91-112和高感TuMV桔红心株系T12-19为亲本建立的小孢子培养DH系作为图谱构建群体, 构建了包含10 个连锁群、406 个标记位点的分子连锁图谱, 图谱总长度826.3 cM, 标记间的平均图距为2.0 cM, 连锁群数目和染色体数相等。每个连锁群上的标记数在7～111个之间, 连锁群的长度在26.4～156.1 cM的范围内, 平均图距在1.0～3.8 cM之间。该连锁图谱包括246个AFLP标记、135个RAPD标记、11个SSR标记和12个同工酶标记、1个SCAR标记和1个形态标记。
|
| 关 键 词： | 大白菜 分子标记 遗传图谱 DH群体 |
| 文章编号： | 0513-353X(2005)02-0249-07 |
| 收稿时间： | 2004-6-2 |
| 修稿时间： | 2004-10-16 |
A Linkage Map Construction for Chinese Cabbage Based on AFLP,SSR,RAPD and Isozyme Markers Using DH Population |
| |
| Zhang Liyang,Zhang Fenglan,Wang Mei,Liu Xiucun,Zhao Xiuyun,Xue Linbao.A Linkage Map Construction for Chinese Cabbage Based on AFLP,SSR,RAPD and Isozyme Markers Using DH Population[J].Acta Horticulturae Sinica,2005,32(2):249-255. |
| |
| Authors: | Zhang Liyang Zhang Fenglan Wang Mei Liu Xiucun Zhao Xiuyun Xue Linbao |
| |
| Institution: | ( National Engineering Research Center for Vegetables, Beijing 100089, China; Horticultural Department, Yangzhou University, Yangzhou 225009, China) |
| |
| Abstract: | A molecular genetic map for Chinese cabbage was constructed based on AFLP (Amplified Fragment Length Polymorphism), RAPD (Random Amplified Polymorphism DNA), SSR (Simple Sequence Repeat) and isozyme markers. Marker analysis was performed on 100 randomly chosen DH lines obtained by microspore culture from the F_1 between two homozygous parents: 91-112 and T12-19. 406 markers including 246 AFLP markers, 135 RAPD markers, 11 SSR markers,12 isozyme markers,1 SCAR and 1 morphological marker were integrated into 10 groups using JoinMap 3.0. It covered 826.3 cM with a mean marker interval of 2.0 cM. Number of markers included in linkage groups varied from 7 to 111, mean marker interval distance from 1.0 cM to 3.8 cM and the length of linkage groups from 26.4 cM to 156.1 cM individually. A total of 45.0% distorted markers distributed in the map. The molecular genetic map constructed in this study would be useful in gene localization, comparative genomes research and QTL mapping of important agronomic traits for Chinese cabbage. |
| |
| Keywords: | Chinese cabbage Molecular marker Genetic linkage map Double haploid(DH) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
