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The occurrence of postharvest gray-mold rot of sweet persimmon caused by Botrytis cinerea in Korea
Authors:Md. Aktaruzzaman  Young-Gyu Lee  Tania Afroz  Byung-Sup Kim
Affiliation:1.National Institute of Crop Science, Highland Agriculture Research Institute, Rural Development Administration,Pyeongchang,Republic of Korea;2.East Coast Life Science Institute,Gangneung-Wonju National University,Gangneung,Republic of Korea;3.Department of Plant Science,Gangneung-Wonju National University,Gangneung,Republic of Korea
Abstract:In this study, we identified the causative agent of postharvest gray-mold rot in sweet persimmon fruit that were collected from Gangneung, Gangwon Province, Korea in October 2016. Symptoms included extensive growth of mycelia on post harvested fruit. The fungus was isolated from infected fruit and cultured on potato dextrose agar (PDA). For identification of the fungus, we examined morphology characteristics and rDNA sequencing analysis of the fungus and confirmed its pathogenicity according to Koch’s postulates. The results of morphological examinations, pathogenicity tests, 5.8S rDNA sequences of the internal transcribed spacer regions (ITS1 and ITS4) and the five nuclear protein-coding genes G3PDH, HSP60, RPB2, MS547 and TUB revealed that the causal agent of postharvest gray-mold rot on sweet persimmon fruit in Korea was Botrytis cinerea.
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