| 茄子及其近缘野生种遗传多样性及亲缘关系的AFLP分析 |
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| 引用本文： | 廖毅,孙保娟,黎振兴,罗少波,陈日远,孙光闻,李植良. 茄子及其近缘野生种遗传多样性及亲缘关系的AFLP分析[J]. 热带作物学报, 2009, 30(6): 781-787 |
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| 作者姓名： | 廖毅 孙保娟 黎振兴 罗少波 陈日远 孙光闻 李植良 |
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| 作者单位： | 华南农业大学园艺学院,广州,510642;广东省农业科学院蔬菜研究所,广州,510640;广东省农业科学院蔬菜研究所,广州,510640;华南农业大学园艺学院,广州,510642 |
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| 基金项目： | 广东省国际科技合作项目,科研条件建设项目,广州市科技攻关重点项目,农业部农业公益性行业科研专项经费项目(No.nyhyzx07-007-14];现代农业技术体系建设专项资金资助 |
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| 摘 要： | 为了明确茄子育种资源的遗传差异和亲缘关系,对来自国内外的54份栽培种茄子高代自交系以及4份近缘野生种进行了遗传多样性的AFLP分析.结果表明:10对EcoRI/MseI引物组合共扩增出570条多态性条带.平均多态率为57%.其中54份栽培种内扩增出120条多态性条带,平均多态率为16%:各引物组合在栽培种内的平均观测等位基冈数、平均有效等位基因数、平均Nei's基因多样度和平均香农信息指数分别为1.176、1.084、0.0051、0.079.基于Jaccard's相似系数采用Complete linkage法进行聚类分析,结果显示:近缘野生种与栽培种的平均遗传距离由近到远依次为红茄、托鲁巴姆、龙葵:栽培种内不同自交系间的相似系数在0.904以上.在相似系数0.915处.栽培种可以分为两个大类;聚类结果与果实性状(果皮色、肉色、果形)和地理来源存在一定的相关性.
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| 关 键 词： | 茄子 遗传多样性 亲缘关系 AFLP |
AFLP Analysis of Genetic Diversity and Genetic Relationship of Eggplant (Solanum melongena L.) and Its Wild Relatives |
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| Liao Yi,Sun Baojuan,Li Zhenxing,Luo Shaobo,Chen Riyuan,Sun Guangwen and Li Zhiliang. AFLP Analysis of Genetic Diversity and Genetic Relationship of Eggplant (Solanum melongena L.) and Its Wild Relatives[J]. Chinese Journal of Tropical Crops, 2009, 30(6): 781-787 |
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| Authors: | Liao Yi Sun Baojuan Li Zhenxing Luo Shaobo Chen Riyuan Sun Guangwen Li Zhiliang |
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| Affiliation: | Liao Yi1,2,Sun Baojuan1,Li Zhenxing1,Luo Shaobo1,Chen Riyuan2,Sun Guangwen2,Li Zhiliang1 1 Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,510640,2 College of Horticulture,South China Agricultural University,510642 |
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| Abstract: | To make clear genetic diversity and relationship among eggplant germplasm, 58 accessions of eggplant including 54 inbred lines and 4 wild relatives were assessed with AFLP markers. The results showed that 570 polymorphic bands were amplified by 10 pairs of EcoRI/MseI primer combinations from all the eggplant germplasm, and that 57% of the germplasm were of polymorphism, of which 54 inbred lines contained 120 polymorphic bands, averaging 16%. The average observed number of alleles, average effective number o... |
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| Keywords: | AFLP |
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