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Expression of proinflammatory cytokine mRNA in the lymphatic organs of adult and neonatal pigs |
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| Authors: | Mikami O Muneta Y Mori Y Yokomizo Y Nakajima Y |
| Affiliation: | a Toxico-pathology Section, Department of Safety Research, National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan b Immunological Mechanism Section, Department of Immunology, National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan |
| Abstract: | Inflammatory cytokine mRNA expression in the lymphatic organs of neonatal, 1-month-old and adult pigs was compared. The mRNA expression of interleukin (IL)-1β, IL-6, IL-18 and tumor necrosis factor (TNF)- in the spleen, thymus, tonsil and popliteal and mesenteric lymph nodes was investigated by semi-quantitative RT-PCR. Stronger IL-1β mRNA expression was observed in the 1-day-old and 1-month-old piglets than in the adult pigs. In thymus, tonsil and mesenteric lymph node, IL-1β mRNA expression in 1-day-old piglets was stronger than in 1-month-old pigs. The expression of IL-6 mRNA in the 1-day-old and 1-month-old tonsil tended to be stronger than in the adult pigs. IL-18 and TNF- mRNA expression was constant in all the samples examined. The expression of IL-1β and IL-6 mRNA may reflect an inflammatory reaction against the exo- and endogenous foreign bodies occurring in the lymphatic organs, especially in the tonsil, of neonatal piglets. |
| Keywords: | Cytokines RT-PCR Lymphatic organ Neonatal pig |
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