麦蛾气味受体OrCo基因的克隆及其表达模式分析

为提高麦蛾茧蜂种群的室内扩繁效率,采用人为控制种蜂婚配条件,研究麦蛾茧蜂交配经历和种蜂性比对交配行为及子代性别分化的影响。结果表明:麦蛾茧蜂种蜂交配经历对雌雄交配行为具有显著影响。交配经历较少的雄蜂再行配对时,求偶次数和交配次数较多、求偶强度较强且单次交配时间较长。没有交配经历的雄蜂比有5~6次交配经历的雄蜂交配时间显著要长。麦蛾茧蜂雄蜂交配经历对子代雌雄性比具有一定影响,但对子代总蜂数量的影响不明显。较少交配经历的雄蜂与处女雌蜂交配繁育所获子代雌蜂数量较多,雌雄性比较高。室内人工繁殖时,适当提高麦蛾茧蜂种蜂中雄蜂的比例有助于提高子代雌蜂数,当种蜂性比为1雌3雄,益害比为2(雌蜂数)10时繁殖效率最佳。

Cloning and expression profile analysis of odorant receptor gene in Sitotroga cerealella

In order to improve the indoor reproductive efficiency of Habrobracon hebetor,effects of mating experience and sex ratio of parent wasps on mating behavior and offspring sex allocation were investigated by controlling the mating condition indoors. Male mating experience had significant effect on the mating behavior of wasps. Male wasps with less mating experience showed more courtship frequency,stronger courtship intensity,more mating frequency and longer mating duration at next mating behavior. The re copulation duration of male wasp without mating experience was significantly longer than that of male wasp with 5 to 6 mating experiences. Male mating experience showed some impacts on the offspring sex ratio,but no obvious impact on the total number of the offspring. When the male with fewer mating experience mated with virgin female,more femalebiased offspring sex ratios were obtained. For indoor rearing,the number of female offspring could be improved by enhancing proportion of the male parent. The best propagating efficiency of H. hebetor was obtained when the sex ratio of parent wasp was 1 female to 3 male,and the ratio of natural enemy to pests was 2 female wasps to 10 5th instar larvae of Plodia interpunctella.