不同年份青砖茶改善小鼠胃肠道功能的研究

以昆明小鼠为对象,研究不同剂量、不同年份湖北青砖茶对小鼠胃排空、小肠推动和肠道菌群作用。理化检测发现,2010年青砖茶氨基酸、茶黄素、茶红素、茶褐素、咖啡碱、有机酸含量显著高于2016年与2006年,儿茶素总量与可溶性糖含量显著低于2016年与2006年。连续给予小鼠青砖茶不同剂量提取物15d后,发现中剂量(1 667mg/kg)改善小鼠肠胃功能的效果最佳,为最有效剂量。高剂量(3 334 mg/kg)减缓胃排空,低剂量(834mg/kg)显著增加双歧杆菌数量,抑制肠球菌数量。另外,与正常组比较,3个年份湖北青砖茶均能有效促进胃排空和肠蠕动,并优化肠道菌群,2006年青砖茶的效果最为显著,主要表现为小肠推进率最高,肠杆菌、肠球菌数量最低,乳酸菌、双歧杆菌数量最高。

Effect of storage time of dark brick tea on improving gastrointestinal function in mice

In this study, we investigated the effect of different dose and storage time of Hubei dark brick tea on gastric emptying, intestinal propulsion and intestinal microflora in Kunming mice. Chemical analysis showed that the contents of free amino acids, theaflavins, thearubigins, theabrownin and coffeine of dark brick tea produced in 2010 were significantly higher, while the contents of total catechins and soluble sugars were significantly lower than the contents of those tea produced in 2016 and 2006. Different dose of dark brick tea extraction were intraperitoneally given to mice for consecutive 15 d, and the medium dose (1 667 mg/kg) could significantly promote gastric emptying and intestinal propulsion in mice, which seemed to be the most effective dose, because high dose (3 334 mg/kg) exhibited an inhibitory effect on gastric emptying and low dose (834 mg/kg) could remarkably increase bifidobaterium and decrease enterococcus. Additionally, in comparison with the control, all the dark brick tea tested improved gastric emptying, intestinal peristalsis, as well as optimized intestinal microflora strikingly. Furthermore, the dark brick tea processed in 2006 was the most effective, with the maximum intestinal propulsion rate, the lowest quantities of enterbacteria and enterococcus, and the highest amount of lactobacillus and bifidobaterium.