| 鱼腥蓝细菌PCC 7120中可控降解系统的构建 |
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| 引用本文： | 王静,张巨源,王莉,陈雯莉. 鱼腥蓝细菌PCC 7120中可控降解系统的构建[J]. 华中农业大学学报, 2018, 37(1): 17-23 |
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| 作者姓名： | 王静 张巨源 王莉 陈雯莉 |
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| 作者单位： | 华中农业大学农业微生物学国家重点实验室，武汉 430070,华中农业大学农业微生物学国家重点实验室，武汉 430070,华中农业大学农业微生物学国家重点实验室，武汉 430070,华中农业大学农业微生物学国家重点实验室，武汉 430070 |
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| 基金项目： | 国家自然科学基金项目（31570048）；中央高校基本科研业务费专项（2014PY003） |
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| 摘 要： | 鱼腥蓝细菌PCC 7120中已有较为成熟的诱导表达系统,但缺乏可控的蛋白降解系统。本研究基于Mesoplasma florum中的Lon蛋白酶(mf-Lon),在蓝细菌中建立可诱导的蛋白降解系统,并通过该系统对关键基因编码产物进行可控降解以研究其生理功能。结果发现降解系统并无预期作用,需进一步改进。
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| 关 键 词： | 鱼腥蓝细菌PCC 7120； 可控蛋白降解系统； mf-Lon蛋白酶； Rnase |
| 收稿时间： | 2017-05-27 |
Construction of controllable protein degradation system in Anabaena sp. PCC 7120 |
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| Abstract: | Anabaena sp. PCC 7120 is an important model strain in the field of cyanobacteria research.Controlling the expression level of gene is one of the most important ways to investigate gene functions.There are a variety of matured gene induction systems available in cyanobacteria. However,no controllable protein degradation system is available in cyanobacteria yet. This work is based on the protein quality control system in Mesoplasma florum and aims to construct an inducible protein degradation system facilitating the investigation of essential genes in Anabaena PCC 7120. Results showed that the degradation or decrease of the target proteins was not detected.It is indicated that the constructed system needs to be further improved. |
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| Keywords: | Anabaena sp. PCC 7120 controllable protein degradation system mf-Lon protein RNase |
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