2种大豆蛋白替代鱼粉蛋白对鲤蛋白酶和淀粉酶活力的影响

为研究不同大豆蛋白源对鲤(Cyprinus carpil)蛋白酶活力和淀粉酶活力的影响,将初始体质量为(10025±098)g的健康鲤180尾随机分为3组,每组3次重复,以鱼粉为动物蛋白源(对照组),全脂豆粉、去皮豆粕为植物蛋白源(全脂豆粉和去皮豆粕分别替代20％鱼粉蛋白),配制3种等蛋白(36％)、等能(15.2 MJ／kg)的半精制饲料,在室内单循环控温养殖系统中进行为期6周的饲养试验,然后分别采用福林—酚试剂法、碘—淀粉比色法测定鲤肝胰脏和肠道蛋白酶与淀粉酶的活力。结果表明:不同大豆蛋白源对鲤蛋白酶活力影响不同,对淀粉酶活力影响不显著。全脂豆粉组鲤肝胰脏、前肠蛋白酶活力与对照组差异不显著(P>0.05);中肠和后肠蛋白酶活力显著下降(P<0.05)。去皮豆粕组肝胰脏和肠道蛋白酶活力与对照组差异不显著(P>0.05)。2种大豆蛋白源对鲤肝胰脏和前肠、中肠、后肠淀粉酶活力影响不显著(P>0.05)。

Effects of Replacement of Fish Meal with Two Soybean Protein Sources on the Activities of Protease and Amylase in Cyprinus carpio

The effects of different soybean protein in diets on the activities of protease and amylase
in Cyprinus carpio were investigated in this study. A growth trial was conducted on Cyprinus carpio withthe initial weight of (100.25±0.98) g for 6 weeksat controlled temperature in single recirculating system. Fish meal was used as animal protein.180 healthy Cyprinus carpio were randomly allocated to three treatments(3×3).The fish was fed semi synthesized diets of three isonitrogenous (36%)and isoenergetic (15.2 MJ/kg) diets formulated with fish meal as animal protein,fall fat soybean meal and dehulled soybean as plant protein formulated with replacement level 20% fall fat soybean meal and dehulled soybean meal for fish protein, respectively. The activities of protease and amylease in Cyprinus carpio were tested using the Folin phenol reagent method and Iodine starch color methodafter growth experiment. The results showed that the effects of different sourcesof dietary soybean protein on the activities of protease in Cyprinus carpioweredifferent, and the activities of amylease in Cyprinus carpio were not significant. The protease activities in hepatopancreas and anterior intestine had no significant difference compared with that of control groups (P＞0.05) with fallfatsoybean meal replaced fish meal by 20%, mid intestine and posterior intestine were significantly declined compared with that of control groups（P＜0.05）. Whendehulled soybean meal replaced fish meal by 20%,the activities of hepatopancreasand intestinal protease were not significantly different compared with that ofcontrol groups（P＞0.05）. The results of this experiment indicated that the effects of two different soybean protein in diets on the amylease activities in hepatopancreas and anterior intestine, mid intestine and posterior intestine werenot significanct for Cyprinus carpio（P＞0.05）.