| 快中子辐射诱变对绿色木霉产纤维素酶的影响 |
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| 引用本文： | 陈光,徐杨,孙旸,刘洁心,王香琪.快中子辐射诱变对绿色木霉产纤维素酶的影响[J].吉林农业大学学报,2011,33(2):177-180. |
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| 作者姓名： | 陈光 徐杨 孙旸 刘洁心 王香琪 |
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| 作者单位： | 吉林农业大学生命科学学院,长春,130118 |
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| 基金项目： | 吉林省科技发展重点项目(20100226) |
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| 摘 要： | 利用快中子对绿色木霉AS3.3711进行辐照,辐照剂量为0.6～4.8 Gy.研究不同剂量快中子对绿色木霉孢子致死率和遗传稳定性的影响,得到了提高纤维素酶活性的菌株.结果表明:辐照剂量在0.6～4.8 Gy 范围内绿色木霉的致死率呈逐渐上升趋势,辐照剂量为1.2 Gy时,绿色木霉的致死率达到80.0%,此突变株的羧甲基...
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| 关 键 词： | 绿色木霉 纤维素酶 快中子 |
| 收稿时间： | 2010-11-17 |
Effect of Fast Neutron Irradiation on Production of Cellulase from Trichoderma viride |
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| CHEN Guang,XU Yang,SUN Yang,LIU Jie-xin,WANG Xiang-qi.Effect of Fast Neutron Irradiation on Production of Cellulase from Trichoderma viride[J].Journal of Jilin Agricultural University,2011,33(2):177-180. |
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| Authors: | CHEN Guang XU Yang SUN Yang LIU Jie-xin WANG Xiang-qi |
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| Institution: | CHEN Guang,XU Yang,SUN Yang,LIU Jie-xin,WANG Xiang-qiCollege of Life Science,Jilin Agricultural University,Changchun 130118,China |
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| Abstract: | The effect of deuterium ion bombardment majority targeted toproduce 14Mev fast neutron irradiation, with dosage 0.6—4.8Gy, on production of cellulose from Trichoderma viride AS3.3711 was studied. The death rate was in a rising trend when irradiation dosage was 0.6—4.8 Gy. The results indicated the death rate of spores reached 80.0％ when radiation dosage was 1.2 Gy. It was shown that one positive mutant had significant difference compared to the originalwith paired samples test (t-test). The filter paper activity of cellulase of strain Fn10-1 reached 633.63 U／mL, with 4 times enhanced, compared with the initial strain. And the CMC activity of cellulase of strain Fn10-1 reached 914.4 U／mL, with 30 times enhanced, compared with the initial strain. |
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| Keywords: | fast neutron |
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