影响降解菌W2修复异噁草酮污染土壤的三种因子的优化

［目的］ 确定降解菌W2对土壤中异噁草酮的最优生物修复条件。［方法］ 采用3因素5水平正交旋转组合设计，室外盆栽生物测定方法，研究降解菌W2接种量、土壤含水量和肥料添加量3种田间可控因子对降解菌W2修复异噁草酮污染土壤效果的影响。［结果］ 确定修复条件的优化数学回归模型为:y=62.363 9+5.872 8×C1-4.494 1×C2C3-1.262 1×C21-4.076 7×C22,不同因子对土壤修复影响大小顺序依次为土壤含水量、肥料添加量、降解菌W2接种量。［结论］ 降解菌W2对土壤中异噁草酮的最优生物修复条件为：降解菌W2接种量8.19～11.81 mL/kg（A650=0.4），土壤含水量18.6%～20.84%，肥料添加量1.83～2.52 g/kg。在此范围内降解菌W2对异噁草酮有效成分浓度为500 μg/kg的风干土壤30 d后的降解率可达60%以上，可接近该修复天数的理论极值65.56%。

Optimization of three factors affecting clomazone degradation in contaminated soils by degrading bacteria W2

Objective] To optimize the conditions for clomazone degradation in contaminated soils by degrading bacteria W2.Method] Three factors affecting degradation rate were studied,including the soil moisture content,the amount of inoculation and fertilizer used.Based on the bioassay method,the degradation rates of bacteria W2 under different conditions were compared by using orthogonally rotational combination design.Result] Mathematical regression model for clomazone degradation was established as follows: y=6...