Development of monoclonal antibodies against the abnormal prion protein isoform (PrPres) associated with chronic wasting disease (CWD) |
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| Authors: | Hyun-Jeong Jeong Nak-Hyung Lee Joong-Bok Lee Seung-Yong Park Chang-Seon Song Kun-Ho Seo Dong-Woon Kim Yong-Sun Kim In-Soo Choi |
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| Affiliation: | 1Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea.;2Department of Public Health, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea.;3Swine Science Division, National Institute of Animal Science, Rural Development Administration, Cheonan 331-801, Korea.;4Ilsong Institute of Life Science, Hallym University, Anyang 431-060, Korea. |
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| Abstract: | Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrPres) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to recognize proteinase K-treated brain homogenate derived from an elk suffering from CWD. |
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| Keywords: | CWD elk monoclonal antibody prion PrPres |
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