双峰驼假结核棒状杆菌病的间接红细胞凝集试验诊断

用间接红细胞凝集试验测定了以假结核棒状杆菌人工感染的1峰和本茵甲醛明矾菌免疫的6峰双峰驼的血清抗体,并绘制了产生的曲线。注射前抗体滴度均在1∶20以下,注射后约20天抗体滴度最高,有些达到1∶1280或1∶2560。以后滴度逐渐降低。6个月以后降到1∶20或更低。血清的第一管稀释度为1∶20。临床健康的双峰驼血清检查了69份。其中1份是曾患假结核棒状杆菌病痊愈者,抗体滴度为1∶80,另2份滴度为1∶20。其余66份均在1∶20以下。并检查本病患驼的血清10份,其中9份为1∶80或更高,1份为1∶40。因此初步提出间接红细胞凝集试验诊断双峰驼假结核棒状杆菌病的判定标准:抗体滴度1∶20为阴性,1∶40为可疑,1∶80为阳性,说明用本法进行诊断本病是快速而可靠的。

Diagnosis of Corynebacterium Pseudotuberculosis Infection in Bactrian Camel with Indirect Hemoagglutination Test

This report indicated that indirect haemagglutination test used for diagnosis of corynebacterium pseudotuberculosis infection of the bactrian camel was a rapid and relevant method. The immunized and infected camels were sampled every ten days intervals from January to July, 1984. The curve of serum antibody production were detected. The results showed that about 20 days after immunization or infection the serum antibody titer was the highest. Till the fourth month the titer decreased to 1:80 or hight higher, and it fell to 1:20 or lower after the sixth month.It was demonstrated that the sixth month postinfection was the appropriate period for examination of serum samples. Using the abovementioned method we suggest that a titer of 1:20 may be assigned as negative result, while 1:80 positive and 1:40 doubtful.