新疆枸杞多糖提取与脱色工艺的研究

[目的]优化新疆枸杞多糖的提取与脱色的工艺条件。[方法]采用单因素试验和正交试验,研究影响枸杞多糖得率的4个因素,确定枸杞多糖提取的最佳工艺条件;采用单因素试验,确定大孔树脂型号,研究影响枸杞多糖脱色的3个因素,确定枸杞多糖溶液脱色的最佳条件。[结果]多糖提取试验中,影响枸杞多糖得率的因素顺序为浸提温度浸提时间浸提次数料液比;最佳提取条件为:料液比1∶20,浸提温度80℃,浸提次数3次,浸提时间3 h。糖溶液脱色试验中,AB-8大孔树脂的脱色效果最好;温度是影响多糖溶液脱色的主要因素,其次为料液比和脱色时间;最佳脱色条件为:温度60℃,料液比1∶7,脱色时间3 h。[结论]该研究为枸杞资源的开发利用提供参考数据。

Extraction and Decolouration Technology for Polysaccharide from Lycium barbarum in xinjiang

[Objective] The study aimed to optimize the technology conditions for the extraction and decolouration of the polysaccharide from Lycium barbarum in Xinjiang.[Method] The single factor experiment and orthogonal test were used to study 4 factors influencing the yield of the polysaccharide from L.barbarum and confirm the optimum technology conditions for extracting the polysaccharide from L.barbarum.The single factor experiment was used to determine the model of the macroporous resin,study 3 factors influencing the decolouration of the polysaccharide from L.barbarum and confirm the optimum conditions for the decolouration of the polysaccharide solution from L.barbarum.[Result] In the polysaccharide extraction test,the effects of 4 factors on the yield of the polysaccharide from L.barbarum were in order of extraction temperature > extraction time > extraction times > ratio of material to liquid;the optimum extracting conditions were as follows: the material-liquid ratio of 1 ∶20,extraction temperature of 80 ℃,extraction times of 3 and extraction time of 3 h.In the decolouration test of the polysaccharide solution,AB-8 macroporous resin got best the decolouration effect;the temperature was the main factor to influence the decolouration of the polysaccharide solution from L.barbarum and followed by the material-liquid ratio and decolouration time;the optimum decoloration conditions were that the decoloration temperature was 60 ℃,the material-liquid ratio was 1 ∶7 and the decoloration time was 3 h.[Conclusion] The study provided the reference data for the development and utilization of the L.barbarum resource.