基于响应面分析法的芹菜黄酮提取优化工艺研究

目的]优选芹菜黄酮的最佳提取工艺条件。方法]以芹菜黄酮提取率为指标,通过析因试验得出影响黄酮提取率的主要因子,在此基础上进行最陡爬坡试验,然后采用响应面分析法对芹菜黄酮提取条件进行优化,确定最佳工艺条件。结果]当乙醇浓度为80%,提取温度55℃时,芹菜黄酮的提取率接近最佳点;确定的最佳工艺条件为料液比1∶15(g/ml),乙醇浓度81%,提取时间3.0 h,提取温度57℃;在此条件下,黄酮提取率可达5.81%。结论]该方法优选出了芹菜黄酮的最佳提取条件,为芹菜资源的深加工提供了理论依据。

Optimization of the Extraction Conditions of Celery Flavonoids by Response Surface Methodology

Objective] To find out the best conditions for extracting celery flavonoids.Method] With the extraction rate of celery flavonoids as the index,the main impact factors on the extraction rate of flavonoids were obtained through factorial experiment,based on which the steepest ascent test was carried out,then the response surface methodology was adopted to optimize the extraction conditions of celery flavonoids and determine the best extraction conditions.Result] When ethanol concentration was 80% and extraction temperature was 55 ℃,the extraction rate of celery flavonoids approached the maximum;the determined best extraction conditions were 1∶ 15(g/ml) solid-liquid ratio,81% ethanol concentration,3.0 h extraction time and 57 ℃ extraction temperature,under those conditions,the extraction rate of flavonoids could reach 5.81%.Conclusion] The method found out the best extraction conditions for celery flavonoids and provided theoretical basis for the deep processing of celery resources.