双酶水解制备大豆玉米活性多肽的研究

[目的]研究双酶水解大豆玉米分离蛋白以制备大豆玉米活性多肽的最佳试验条件。[方法]以大豆蛋白和玉米蛋白为主要原料,配制不同浓度的大豆玉米分离蛋白溶液,经预处理后,分别用风味蛋白酶、复合蛋白酶、中性蛋白酶、木瓜蛋白酶和菠萝蛋白酶水解,通过测定水解度和酶活力,确定试验用酶。并将所选酶按一定比例两两混合,采用正交试验确定双酶水解的最佳条件。[结果]最佳水解条件为大豆玉米分离蛋白溶液浓度2%,风味蛋白酶与复合蛋白酶的比例1∶1,pH 7.0,水解时间8 h;在该条件下,大豆玉米分离蛋白的水解度可达75.14%。[结论]该研究为大豆玉米活性多肽的开发与应用奠定了基础。

Preparation of Active Peptides from Corn and Soybean by Bi-enzyme Hydrolysis

[Objective] The aim was to study the optimal test condition for preparation of active peptides from corn and soybean by bi-enzyme hydrolysis.[Method] Taking soybean peptide and corn peptide as materials,different concentrations of soy-corn protein isolate solution were prepared.Through pretreatment,and based on compound protease and flavor protease,neutral protease,papaya protease,pineapple hydrolysis,and by measuring the degree of hydrolysis activity and enzyme activity,tested enzyme was determined.Mixing two of those enzymes in certain proportion and by orthogonal test,the optimal condition of enzyme hydrolysis of a double was determined.[Result] The optimal hydrolysis condition was 2% of substrate concentration,1∶ 1 of hydrolyze compound protease and flavor protease,and 8 h of hydrolysis time,75.14% of DH.[Conclusion] The study provided a basis for the development and application of active peptides from corn and soybean by bi-enzyme hydrolysis.