马铃薯脱病毒与快繁技术研究

目的]获得马铃薯脱毒试管苗及使长期保存导致生长势退化的试管苗复壮。方法]设置不同的灭菌方法和不同的培养基进行马铃薯茎尖脱病毒及脱毒苗的快繁技术研究。结果]芽外植体灭菌效果以质量分数5%的NaClO灭菌时间2 min与0.1%的HgCl2灭菌时间3 min组合效果最佳;茎尖诱导的最佳培养基为MS+GA30.1 mg/L+6-BA 0.5 mg/L+IBA 0.2 mg/L,平均诱导率为58.25%;从MS液体和MS固体2种不同的培养基来看,MS液体培养基可以起到壮苗的作用;从扦插和平铺2种不同接种方式来看,平铺的接种方式可以加快脱毒苗繁殖速度。结论]为马铃薯的组织培养快繁提供了参考依据。

Study on Virus-free and Rapid Propagation Technique of Potato

Objective] The study aimed to get the virus-free tube plantlets of the potato and rejuvenat the tube plantlets that had the growth potential degradation in the long-term subculture.Method] The different sterilization methods and media were set up to carry out the study on the virus-free and rapid propagation technique of the potato.Result] The combinations of 5% NaClO for 2 min and 0.1% HgCl2 for 3 min could get the optimum sterilization effects on the bud explants of the potato.The optimum medium for the induction of the shoot tip was MS+GA3 0.1 mg/L+6-BA 0.5 mg/L+IBA 0.2 mg/L,with the mean induction rate of 58.25%.Comparison between the 2 media of MS liquid and MS solid showed that MS liquid medium had the action of rejuvenating the tube plantlets.Comparison between the different inoculation patterns of the cutting propagation and the horizontal propagation indicated that the horizontal inoculation pattern could accelerate the reproductive speed of virus-free tube plantlets.Conclusion]The study provided the reference basis for the tissue culture and rapid propagation of the potato.